RNA干扰Fas基因表达的免疫组化半定量分析  

Issue of Effects of RNAi Inhibited Fas Expression Via Semi Quantitative Analysis of Immunohistochemical Image

作　　者：刘明社[1] 赵中夫[2] 张国英[2] 张芸[2] 武延隽[1] 

机构地区：[1]长治医学院人体寄生虫学教研室,046000 [2]长治医学院传染病学教研室

出　　处：《长治医学院学报》2007年第6期404-406,共3页Journal of Changzhi Medical College

基　　金：山西省自然基金资助项目(20051114)

摘　　要：目的:探索图像分析技术在Fas蛋白表达检测中的作用。方法:用Image Pro Plus(IPP)软件图像半定量分析技术,判定RNA干扰抑制Fas基因后小鼠肝组织免疫组织化学染色切片和Western Blot检测的Fas蛋白表达情况。结果:RNA干扰有效抑制了Fas基因的表达,IPP图像分析方法显示,Fas蛋白表达在免疫组织化学染色切片的差异与Western Blot检测结果一致。结论:Image Pro Plus图像分析手段有利于通过半定量的方式判定Fas蛋白表达的变化。Objective： It is surveyed the effect of image analysis in detecting expression of Fas protein. Methods：Image Pro Plus（IPP） was used as the method of semi- quantitative image analysis on immunohistochemical slide image and on Western Blot specific protein strip image, and evaluating the expressing protein of Fas gene inhibited by RNAi. Results ： Statistical analysis showed that expression of Fas gene effectively inhibited by RNAi. The result of semi quantitative image analysis on immunohistochemical slide image accorded with the result of on Western Blot specific protein strip image. Conclusion：Image Pro Plus is useful method in analyzing the immunohistochemical images of Fas protein expression.

关 键 词：RNA干扰 FAS蛋白 免疫组织化学 图像分析 

分 类 号：R392.31[医药卫生—免疫学]