传染性支气管炎重组鸡痘病毒免疫鸡对LHLJ04XI株病毒攻击的免疫保护作用  

Protection of chickens against IBV field strain with a recombinant fowlpox virus co-expressing IBV S1 gene and ChIFNγ gene

作　　者：石星明[1] 王云峰[1] 王玫[1] 刘胜旺[1] 童光志[1] 何来[1] 贺笋[1] 崔红玉[1] 胡文玮[1] 韩文雄[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2008年第1期47-52,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家“863”计划资助项目(2003AA213020)

摘　　要：将表达鸡IFN-γ基因和传染性支气管炎病毒S1基因的重组鸡痘病毒(rFPV-IFNγ S1)接种SPF鸡后,用与S1基因亲本毒株具有相同致病型且遗传关系较近的现地分离株LHLJ04XI病毒进行攻击,评价重组疫苗对现地分离病毒的保护效果。结果显示,重组病毒在免疫后1周即可检出ELISA抗体,并且免疫组外周血CD4+、CD8+T淋巴细胞含量均有显著上升。当用LHLJ04X I株病毒及亲本毒LX4攻击后,免疫鸡的肝脏、肾脏、脾脏及肺脏等脏器的病理损伤程度明显轻于非免疫对照组,排毒时间缩短,攻毒后现地分离株攻击的免疫组的发病率为27.3%(3/11),死亡率为9.1%(1/11),亲本毒攻击的免疫组发病率为26.67%(4/15),死亡率为6.67%(1/15),两者没有明显差异,而非免疫对照组的发病率和死亡率分别为100%(11/11)和45.5%(5/11),与免疫组相比差异显著,说明重组疫苗对免疫鸡具有较好的保护效果。体重测定结果表明,攻毒前重组疫苗免疫组和非免疫对照组的体重差异不显著(P>0.05),攻毒以后免疫组体重的增加幅度高于非免疫对照组。以上结果表明,共表达鸡IFN-γ基因和传染性支气管炎病毒S1基因重组鸡痘病毒活载体疫苗能对异源现地分离株的攻击产生较好的免疫保护,同时也减轻了重组疫苗对体重增长的抑制作用。To investigate protective efficacy of the fowlpox virus vaccine co-expressing infectious bronchitis virus （IBV） S1 gene and chicken interferon-γ gene （rFPV-IFN γ S1）, four-week old SPF chickens were inoculated with rFPV-IFN γ S1 vaccine and challenged with IBV field strain LHLJ04XI and parental IBV LX4 three weeks post-inoculation. The results showed that chickens immunized with rFPV-IFN γ S1 developed IBV specific serum antibodies one week after immunization. The immunized chickens showed less pathological changes and shorter period of virus shedding, with less than 10% motality after challenged with LHLJ04XI and parental IBV LX4. In contrast, the control group showed 100 % （11/11） morbidity and 45.5 % （5/11） mortality. Bodyweight analysis showed a higher weight gains for chickens in the immunized group. In conclusion, rFPV-IFN γ S1 could effectively induce protection against heterologous IBV field strain LHLJ04XI challenge, and alleviate the affect of recombinant virus immunization on chicken weight gains.

关 键 词：传染性支气管炎 鸡痘病毒 免疫保护 

分 类 号：S852.659[农业科学—基础兽医学]