硼对不同硼效率甘蓝型油菜叶片6-磷酸葡萄糖脱氢酶活性和可溶性糖含量的影响  

Effects of Boron on Glucose-6-phosphate Dehydrogenase Activity and Soluble Sugar Content in the Leaves of Rape Cultivars(Brassica napus L.) with Different Boron Efficiency

作　　者：年夫照[1] 胡承孝[1] 徐芳森[1] 吕芬[2] 石磊[1] 王运华[1] 

机构地区：[1]华中农业大学微量元素研究中心,湖北武汉430070 [2]云南农业大学烟草学院,云南昆明650201

出　　处：《云南农业大学学报》2008年第1期47-51,共5页Journal of Yunnan Agricultural University

基　　金：国家自然科学基金项目(30170549);云南农业大学人才引进项目(A2002112)。

摘　　要：通过营养液培养试验,研究了硼对不同硼效率甘蓝型油菜品种叶片6-磷酸葡萄糖脱氢酶(G-6-PDHase)活性和可溶性糖含量的影响。结果表明,在正常硼营养条件下,随着生育期的推进,叶片酶活性先升高,在花期达到顶点,而后下降,可溶性糖也有类似的变化。在升高和下降的快慢上,高效品种要大于低效品种。低硼胁迫使两油菜品种叶片可溶性糖含量和酶活性均有不同程度的升高。对于可溶性糖,低效品种在低硼处理下的叶片累积量明显高于其他处理,高效品种低硼处理次之,高效品种硼正常处理最低;酶活性上,在苗期和蕾薹期,低效品种升高的幅度大于高效品种,而在花期及其以后时间内,则高效品种升高的幅度要大于低效品种。By solution culture, the effects of boron （B） on Glucose-6-phosphate dehydrogenase （G-6-PDHase） activity and the soluble sugar in leaves of rape cultivars （ Brassica napus L. ） with different B efficiency were studied. The results showed that with the progress of growth period, the enzyme activity in the leaves, as well as the soluble sugar, increased firstly, then decreased after flowering, and that the change extent in high B efficiency cuhivar was higher than that in low B efficiency cultivar. Under low B stress condition, the soluble sugar and the enzyme activity in the leaves of the two rapeseed cultivars all increased. The soluble sugar content in the leaves of low B efficient cuhivar un- der low B stress was the highest among all treatments, followed by high B efficient cultivar under the same condition, and the lowest was high B efficient cuhivar under optimum B level. For enzyme activity, the increasing extent of low B efficient cultivar was higher than that of high B efficient cuhivar in seedling or budding, and the cultivar was reversed after flowering.

关 键 词：甘蓝型油菜 硼 硼效率 可溶性糖 6-磷酸葡萄糖脱氢酶 

分 类 号：S565.401[农业科学—作物学]