磁性附着体模拟静磁场对成骨细胞形态和表面超微结构的影响  

Effect of simulating static magnetic field of magnetic attachment on osteoblastic morphology and surface ultrastructure

作　　者：姚蔚[1] 赵煜[3] 李冰雁[2] 巢永烈[1] 杜莉[1] 

机构地区：[1]四川大学华西口腔医学院修复科,成都610041 [2]四川大学华西口腔医学院颞下颌关节科,成都610041 [3]中山大学光华口腔医学院修复科

出　　处：《中华口腔医学杂志》2008年第1期48-49,共2页Chinese Journal of Stomatology

基　　金：四川省应用基础研究计划（02sy029.138）

摘　　要：目的研究磁性附着体模拟的静磁场对成骨细胞形态和表面超微结构的影响，以期逐步了解磁性附着体的生物学效应。方法对体外培养的SD大鼠成骨细胞施加12．5、125和250mT的静磁场，加载1、3、5、7d，对照组不加载静磁场，观察细胞形态、排列及表面超微结构。结果各组细胞生长良好，均未见静磁场对细胞形态、排列有影响。125mT和250mT静磁场加载3d，12．5mT静磁场加载5d后，细胞表面出现较多的微囊泡。结论静磁场加载对成骨细胞的形态、排列无明显影响；静磁场加载后细胞表面超微结构发生改变。Objective To investigate effects of the static magnetic field （SMF） generated by dental magnetic attachment on osteoblastic morphology and surface ultrastructure. Methods The in vitro cultured rat osteoblasts were exposed continuously to 12.5 mT, 125 mT, and 250 mT static magnetic fields for 1, 3, 5, and 7 days. After exposed in SMF, osteoblasts were observed under a phase contrast microscope, and then HE stained and observed under a light microscope. In addition, the cells were observed under a scanning electron microscope （SEM）. Results By continuous exposure, the different intensities of SMF exposure did not change the vital osteoblast growth pattern or distribution. The SEM photos showed that there were certain changes in cellular microstructures for osteoblasts after exposed to 12.5 mT for 5 to 7 days, as well as 125 mT and 250 mT for 3 to 7 days. The more exposure time increased, the more microvesicles on the surfaces of cells were observed. Conclusions Continuous SMF-stimulation could not affect the shape, distribution, and growth pattern of osteoblasts. The SMF of magnetic attachments could lead to certain changes in surface uhrastructures of osteoblasts in this study.

关 键 词：成骨细胞 义齿精密附着体 静磁场 超微结构 

分 类 号：R686[医药卫生—骨科学]