人牙周膜成纤维细胞对四环素的跨膜转运  

Biological transport of tetracycline hydrocldoride by human periodontal ligament fibroblasts

作　　者：刘宇[1] 刘洪臣[1] 吴霞[1] 鄂玲玲[1] 冷斌[1] 

机构地区：[1]解放军总医院口腔医学研究所,北京100853

出　　处：《中华口腔医学杂志》2008年第1期57-58,共2页Chinese Journal of Stomatology

摘　　要：目的研究人牙周膜成纤维细胞（human periodontal ligament fibroblasts，HPDLF）对四环素的跨膜转运，为通过根管局部或全身给药假说提供实验依据。方法盐酸四环素溶液孵育HPDLF和MC3T3-E1细胞，超声破碎细胞后，高效液相色谱法测定胞内药物含量，考马斯亮蓝法测定细胞蛋白质量。结果10mg／L四环素孵育1、5、10min后细胞内四环素含量与细胞蛋白质量的比值分别为（0．192±0．008）、（0．212±0．082）、（0．620±0．075）ng／μg。20mg／L四环素孵育5min后细胞内四环素含量与细胞蛋白质量的比值为（0．503±0．056）ng／μg。10mg／L四环素孵育5min后MC3T3-E1细胞内四环素含量与细胞蛋白质量的比值为（0．666±0．560）ng／μg。结论HPDLF可跨膜转运四环素。转运与孵育时间及细胞外药物浓度相关。Objective To investigate biological transport of tetracycline hydrochloride by human periodontal ligament fibroblasts （HPDLF） for verifying the hypothesis of delivering medicine to the periodontium and whole body through the root canal. Methods HPDLF and MC3T3-E1 ceils were incubated in antibiotics solutions. The intracellular antibiotics contents were measured by high performance liquid chromatography （HPLC） and the cell total protein was measured by bradford protein assay. Results The intracellular contents increased with incubation time. The extracelluiar medicine concentration had effect on the intracellular contents. Conclusions Tetracycline hydrochloride can be transported into HPDLF with incubation and this transport is time-dependent and concentration-dependent.

关 键 词：成纤维细胞 四环素 生物转运 

分 类 号：R686[医药卫生—骨科学]