野牛草实生群体多样性的表型及ISSR分析  

Analysis of genetic diversity within the population of buffalo grass by morphological traits and ISSR markers

作　　者：刘莉[1] 邓春婷[2] 包满珠[1] 

机构地区：[1]华中农业大学园艺林学学院园艺植物生物学教育部重点实验室,湖北武汉430070 [2]华中农业大学园艺林学学院,湖北武汉430070

出　　处：《草业科学》2008年第1期100-106,共7页Pratacultural Science

基　　金：国家"863"课目资助(2004AA244050)

摘　　要：从野牛草Biichloe dactyloides种子播种繁殖的102株单株中筛选20株表型有一定差异的单株作为研究对象，利用ISSR分子标记和表型性状研究野牛草群体内的多样性。从50条ISSR引物中筛选出7条适合野牛草的ISSR51物，共扩增出89条带，多态性条带74条，比率为83.14％。经NTSYS软件聚类分析结果表明，野牛草群体内的遗传多样性较高，相似系数为0.58～0.82，遗传距离分布为0.17～0.42。实生苗群体内可以采用单株选育优良无性系，并选择出一些较好的中间育种材料。ISSR标记聚类结果与形态特征的相关系数为0.1976（P〈0.05），没有明确的对应关系，预示选择野牛草杂交亲本时不仅要考虑表型的差异也要顾及分子标记的差异。The aim of this study was to analyse the genetic diversity by ISSR marker and morphological traits within population of 20 Buffalo grass（Biichloe dactyloides） accessions that selected from 102 individuals. Seven ISSR primers were selected from 50 ISSR primers, and 74 （83.1%） polymorphic loci were detected out of 89 loci in total. It showed that the genetic polymorphism among population was high and the coefficient among each accessions ranged from 0.68 to 0.82. The genetic distance ranged from 0.17 to 0.42. The results indicated that individual selection was suitable in Buffalo grass breeding even within seedling population. Dendrogram obtained based on ISSR markers was not in accordance with that on morphologic characteristics, which suggested that not only morphological traits but also molecular genetic basis should be taken into account in selecting parents for Bufflo grass crossing.

关 键 词：野牛草 ISSR实生群体 多样性 表型 

分 类 号：S688.4[农业科学—观赏园艺]