金雀异黄素与5-氟尿嘧啶对SGC-7901细胞的抑制作用  

Effect of Genistein Combined with 5-FU on Human Gastric Cancer Cell Line SGC-7901 in Vitro

作　　者：王正文[1] 朱明才[2] 汤为学[3] 龚军[4] 范维珂[3] 魏锦来[2] 崔海宁[1] 

机构地区：[1]海南医学院附属医院,海口570102 [2]重庆医科大学附属第一医院普外科,重庆400016 [3]重庆医科大学基础医学院病理生理教研室,重庆400016 [4]四川省广安市人民医院普外科,广安638000

出　　处：《中国普外基础与临床杂志》2008年第1期17-22,共6页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的研究金雀异黄素（genistein，Gen）与5-氟尿嘧啶（5-fluorouracil，5-FU）对人胃癌细胞SGC-7901的抑制作用。方法采用MTT法检测Gen和5-FU对SGC-7901细胞的抑制作用，流式细胞术检测细胞周期分布，并在透射电镜下观察细胞超微结构改变。结果Gen和5-Fu单用或联合应用对胃癌SGC-7901细胞的生长均有显著的增殖抑制作用，且呈剂量和时间依赖性。两药联合应用时，当药物效应在0．2～0．8时，具有协同或相加作用（CI≤1）；18．8btmol／L的Gen作用后，62．97％的SGC-7901细胞阻滞在G2／M期；8．84μmol／L的5-FU作用后，63．76％的SGC-7901细胞阻滞在s期；3．06μmol／L的Gen与7．96μmol／L的5-Fu联合应用后，67．46％的SGC-7901细胞阻滞在G0／G1期。Gen和5-FU均可诱导SGC-7901细胞凋亡。结论Gen与5-FU通过阻滞细胞周期和诱导细胞凋亡而抑制胃癌细胞的生长，对胃癌细胞的增殖抑制具有协同作用。Objective To study the effect of genistein （Gen） in combination with 5-fluorouracil （5-FU） on human gastric carcinoma cells in vitro. Methods Human gastric carcinoma cell line SGC-7901 was treated with Gen and 5-FU alone or in combination for 24, 48 and 72 hours, respectively. MTT assay was used to investigate the inhibitory effect of Gen and 5-FU on SGC-7901 cells. Transmission electron microscopy was used to observe the ul- tramicrostructure changes of cancer cells and the flow cytometry was used to detect the distribution of cell cycle. Resuits Gen and 5-FU alone or in combination inhibited the proliferation of SGC-7901 cells significantly in both timedependent and dose-dependent manner. The combination of Gen with 5-FU had synergistic effect on the growth of SGC-7901 cell line when the inhibition ratio was 20% to 80%. When SGC-7901 cells exposed to 18.8 μmol/L Gen, 8.84 9mol/L 5-FU, and 3.06 μmol/L Gen combined with 7.96 μmol/L 5-FU for 72 hours, 62.97%, 63.76% and 67.46 % SGC-7901 cells were arrested in G2/M, S and G0/G1 phrase, respectively. Gen and 5-FU could both induce apoptosis and arrest cell cycle of SGC-7901 cells. Conclusion Combination therapy of Gen with 5-FU may take synergistic inhibitory effect on the proliferation of gastric cancer cells by resting cell cycle and inducing cell apoptosis at a certain range of concentration.

关 键 词：胃癌 SGC-7901细胞 金雀异黄素 5-氟尿嘧啶 抑制作用 

分 类 号：R96[医药卫生—药理学]