异搏停与化疗同时应用对荷瘤小鼠的治疗作用  

Effects of verapamil and chemotherapy on tumor-bearing mice

作　　者：邹大伟[1] 巴静[1] 吉阳涛[1] 路平[2] 

机构地区：[1]中国医科大学附属第一医院检验科,沈阳110001 [2]中国医科大学附属第一医院肿瘤科,沈阳110001

出　　处：《广东医学》2008年第1期39-41,共3页Guangdong Medical Journal

基　　金：辽宁省自然科学基金资助项目(编号:20042071)

摘　　要：目的观察异搏停和化疗药同时应用对荷瘤小鼠的治疗效果,为肿瘤的治疗提供理论依据。方法作荷瘤小鼠动物模型,随机分为4组荷瘤组(LT)组、化疗组(C组)、异搏停组(V组)、化疗药+异搏停组(CV组)癌瘤内注射给药,观察荷瘤小鼠的抑瘤率、肿瘤坏死情况和小鼠病死率,同时用RT-PCR方法测其多药耐药基因(multidrugsresistancegene,MDR1)的表达。结果C组、CV组抑瘤率分别为29.73%,45.95%,两组比较差异有显著性(P<0.05);C组、CV组死亡比例分别为3/20,2/20,与LT组(8/20)比较差异有显著性(P<0.05);CV组肿瘤坏死严重;LT组、C组、V组、CV组的MDR1分别为12/12,12/12,3/12,2/12,V组、CV组与LT组的MDR1比较差异有显著性(P<0.05),LT组与C组的MDR1比较差异无显著性(P<0.05)。结论异搏停和化疗药同时应用较单纯化疗抗肿瘤治疗的效果为佳,提示临床化疗时应给予逆转MDR1的药物,以提高肿瘤的治疗效果。Objective To study the therapeutic effects of verapamll and chemotherapeutic agents on tumor - bearing mice. Methods verapamil and chemotherapeutic agents were injected into tumor centre of tumor - loading mice. The inhibition rate of tumor, pathology of tumor and mortality were examined. The expression of multidrugs resistance gene （MDR1） was examined by RT- PCR. Results The expression of MDR1 in loading tumor group （LT）, chemiotherapy group （C）, verapamil group （V） and chemotherapy verapamil group （CV） was 12/12, 12/12, 3/12 and 2/12 respectively and there was statistical significance between V group and LT group （ P 〈 0.05 ） and between LT group and C group （ P 〈 0.05 ）. Inhibition rate of tumor in C group and CV group was 29.73% and 45.95% respectively, significantly higher than that of LT group （ P 〈 0.05） and V group in （ P 〈 0.05 ） respectively. Mortality in C group （3/20） and CV group （2/20） were significantly lowered compared to LT group （8/20, P 〈 0.05 ）. Tumor necrosis was prominent in CV group. Conclusion Combined verapamil and chemotherapeutic agent is more effective for loading tumor in mice than chemotherapeutic agent alone. Addition of MDR1 reversing drugs enhances the efficacy of chemotherapeutic agent.

关 键 词：癌 异搏停 MDR1 死亡率 抑瘤率 

分 类 号：R285.5[医药卫生—中药学]