食品中产单核细胞李斯特菌PCR检测方法的建立  

Establishment of PCR detection Method for Listeria monocytogenes in Food

作　　者：白艳艳 张彦明[1] 郑增忍[2] 张衍海[2] 龚振华[2] 张宝[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨陵712100 [2]中国动物卫生与流行病学中心,266032

出　　处：《中国动物检疫》2008年第1期29-30,45,共3页China Animal Health Inspection

基　　金：陕西省重大科技专项(2006kz07-G2)

摘　　要：根据产单核细胞李斯特菌hlyA基因设计引物,进行PCR扩增,检测该方法的特异性和灵敏度。人工污染样品经Half-fraser和Fraser增菌后进行PCR检测。结果表明,产单核细胞李斯特菌扩增出234bp的条带,对照菌未扩增出目的条带。该方法的灵敏度为104cfu/mL。人工污染样品的检出限为8cfu/25g,说明PCR方法检测食品中产单核细胞李斯特菌具有快速、特异、敏感等特点,具有较高的实用价值。A PCR was established for the detection of Listeria monoeytogenes （LM） in food with a pair of primers specific to the LM hly A gene. The sensitivity and specificity of this method were detected.. The procedure was validated by detecting Listeria monocytogenes in artificially contaminated food and PCR analysis was taken following Half-fraser and fraser enrichment steps. The specific 234 bp fragment was amplified in LM while none was amplified in all other species. The specificity is 104 cfu /mL.As few as 8 cfu /25 g can be detected in artificially contaminated food. The PCR procedure has proved to be a rapid sensitive and specific method suitable for detecting Listeria monocytogenes in food.

关 键 词：产单核细胞李斯特菌 PCR 检测 

分 类 号：S858.23[农业科学—临床兽医学]