禽流感病毒HA基因在狂犬病毒中的表达  

Recombinant Rabies Virus Expressing Hemagglutinin of Avian Influenza Virus

作　　者：艾军[1] 金晓芹[1] 孙京臣[2] 罗琴芳[1] 黄文科[1] 郭霄峰[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]华南农业大学动物科技学院,广东广州510642

出　　处：《华南农业大学学报》2008年第1期81-83,共3页Journal of South China Agricultural University

基　　金：广东省自然科学基金团队项目(5200638);国家自然科学基金(30671565)

摘　　要：为了研究狂犬病毒作为载体表达外源基因的特性,将禽流感病毒HA基因插入狂犬病毒全基因组cDNA感染性克隆pHEP-3.0G基因与L基因的假基因位点,再通过反向遗传技术,获得了携带流感病毒HA基因的嵌合狂犬病毒(HepFHA),并进行了病毒传代与HA基因表达的研究.结果显示,重组病毒经BHK-21细胞传代10次,病毒表达稳定,但各代次常规红细胞凝集试验均为阴性;Western-blotting检测到病毒表达产物中存在部分HA蛋白的表达,其相对分子质量为39 540,与HA裂解的HA1蛋白相对分子质量相吻合.To study the characteristics of rabies virus as vector, hemagglutinin gene of avian influenza virus was inserted at the site of pseudogene between G and L genes and a chimeric virus, designated as HepFHA, was generated by the technique of reverse genetics. The virus HepFHA grew well in BHK-21 cells after passage for 10 times, but did not show hemagglutination. The foreign protein was analyzed by SDS-PAGE and Western-blotting, and the results indicated that the partial protein of hemagglutinin was expressed with relative molecular mass of 39 540, identical to that of HA1.

关 键 词：狂犬病毒 反向遗传技术 HA基因 

分 类 号：S852.6[农业科学—基础兽医学]