用亲和层析法分离纯化降纤酶  

Purification of Defibrase with Afinity Chromatography

作　　者：黄寓[1] 金星光[1] 侯庆华[1] 

机构地区：[1]辽宁省血栓病中西医结合医疗中心研究所,辽宁沈阳110101

出　　处：《中国药业》2008年第2期26-27,共2页China Pharmaceuticals

摘　　要：目的用亲和层析法从尖吻蝮蛇蛇毒中分离纯化降纤酶。方法用离子交换色谱、单克隆抗体亲和色谱技术进行分离纯化。结果得到电泳纯的降纤酶,其分子量为36000。结论用单克隆抗体亲和色谱法纯化降纤酶是切实可行的。Objective To purify defibrase from snake venom of Agkistrodon acutus by affinity chromatography. Methods Ion exchange chro- matography and monoclonal antibody affinity chromatography were used. Results The purified defibrase showed one band in SDS- PAPGE with relative molecular weight 36KD. Conclusion The method of the monoclonal antibody affinity chromatography is a practicable production procedure.

关 键 词：蛇毒 降纤酶 亲和层析 

分 类 号：TQ461[化学工程—制药化工] R996.3[医药卫生—毒理学]