Strain Mesorhizobium sp.CCNWGX035:A Stress-Tolerant Isolate from Glycyrrhiza glabra Displaying a Wide Host Range of Nodulation  

作　　者：WEI Ge-Hong YANG Xue-Ying ZHANG Zhi-Xin YANG Ya-Zhen K. LINDSROM 

机构地区：[1]Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Life Science, Northwest A＆F University, Yangling Shaanxi 712100 （China） [2]Department of Applied Chemistry and Microbiology, Biocenter 1, Fin-0014 University of Helsinki （Finland）

出　　处：《Pedosphere》2008年第1期102-112,共11页土壤圈（英文版）

基　　金：Project supported by the Program for the New Century Excellent Talents in University of China (No.NCET-04-0953);the Fund of Author of Chinese Excellent Doctor Degree Dissertation (No.200254);the National Natural Science Foundation of China (Nos.30470040 and 30670372).

摘　　要：Thirty-nine rhizobial isolates were isolated from the root nodules of Glycyrrhiza uralensis and Glyeyrrhiza glabra, growing in the arid and semiarid regions of northwestern China, to test their taxonomic position and stress tolerance and to select one promising putative inoeulant strain for further studies. On the basis of 113 physiological and biochemical characteristics, the isolates were clustered into three groups. One isolate CCNWGX035 was found to have high tolerance to NaC1, pH, and temperature. By sequencing the 16S rDNA, isolate CCNWGX035 was placed in genus Mesorhizobium. Nodulation tests demonstrated that the isolate not only formed nitrogen-fixing nodules on its original host plant Glyeyrrhiza glabra, but also on Sophora vieiifolia, Lotus cornieulatus, Trifolium repens, Melilotus suaveolens, and Sophora alopecuroides. On the basis of sequence analysis of the nodA gene, isolate CCNWGX035 was closely related to strains of the genus Mesorhizobium, exhibiting some novel characteristics of root nodule bacteria.39 rhizobial 孤立从 Glycyrrhiza uralensis 和 Glycyrrhiza glabra 的根瘤被孤立，在西北的中国的干旱、半干旱的区域成长，测试他们的分类位置和压力忍耐并且为进一步的研究选择一有希望的通常认为的种体紧张。根据 113 生理、生物化学的特征， isolates 被聚类进三个组。一个孤立 CCNWGX035 被发现有高忍耐到 NaCl， pH，和温度。由定序 16S rDNA，孤立 CCNWGX035 被放在类 Mesorhizobium。生节测试证明 isolate 它的原来的寄植物 Glycyrrhiza glabra 上，而且在 Sophora viciifolia，莲花 corniculatus， Trifolium 钢笔， Melilotus suaveolens，和 Sophora alopecuroides 上的不仅形成的修理氮的小瘤。根据点头的顺序分析基因，孤立 CCNWGX035 仔细与类 Mesorhizobium 的紧张有关，展出根瘤菌的一些新奇特征。

关 键 词：GLYCYRRHIZA host range MESORHIZOBIUM nodA stress tolerance 

分 类 号：S15[农业科学—土壤学]