预先鞘内注射氯诺昔康对福尔马林致痛大鼠行为学及脊髓c-Fos蛋白表达的影响  

Effects of Preemptively Injected Intrathecal Lornoxicam on Behavior and c-Fos Protein Expression of Rat with Formalin Hurting

作　　者：陈旭[1] 杨勇 刘慧[1] 肖红[1] 杨邦祥[1] 

机构地区：[1]四川大学华西医院麻醉与危重病学实验室 [2]四川省红十字骨科医院骨科

出　　处：《四川大学学报（医学版）》2008年第1期89-93,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的通过观察比较福尔马林致痛大鼠行为学和脊髓背角c-Fos样免疫反应神经元(FLIN)数量的变化,探讨预先鞘内注射氯诺昔康的镇痛效能与其剂量的关系。方法雄性SD大鼠42只,随机平均分为7组,生理盐水组(NS组)、福尔马林组(FOR组)和5个实验组(L60组、L120组、L180组、L240组、L300组),行清醒鞘内穿刺,注入NS或不同剂量的氯诺昔康(60μg、120μg、180μg、240μg、300μg),10min后足底皮下注射NS或5%福尔马林100μL,观察行为表现,计算5min内伤害反应指数(NBI),连续60min。足底注射后2h取脊髓L4～L6节段做c-Fos免疫组化染色,计数FLIN数量。结果1第一相(致痛后0～5min)NBI,L240、L300组较其他组低(P<0.01),二者组间差异无统计学意义。2随着氯诺昔康剂量的增加,第二相(致痛后15～60min)NBI累加值有下降的趋势,但L180～L300三组间比较差异无统计学意义。3随着氯诺昔康剂量的增加,致痛侧脊髓背角浅层和深层FLIN数量有下降的趋势,但L180～L300三组的浅层及L240组、L300组的深层FLIN数量比较差异均无统计学意义。4大鼠1h的NBI累加值与致痛侧脊髓背角浅层和深层c-Fos蛋白表达的相关系数分别为0.865、0.855(P均<0.001)。结论预先鞘内注射氯诺昔康能有效抑制大鼠的伤害性行为和致痛侧脊髓背角c-Fos蛋白的表达,且该作用与剂量有关,并存在封顶效应。Objective The objective of this study was to investigate an antinociceptive effect of preemptive intrathecal lornoxicam on behavior and expression of c-Fos protein in the spinal cord of the formalin hurting rats. Methods Forty-two healthy male adult SD rats （200-250 g） were randomized into 7 groups. In the normal saline control group （group NS）, 20 μL of normal saline was injected intrathecally, which was followed by a subcutaneous administration of 100 μL of normal saline into the hind plantar skin of rats 10 min later. In the formalin control group （group FOR）, 20μL of normal saline was injected intrathecally, and followed by rat intraplantar administration of 100 μL of 5% formalin. In the intrathecal lornoxicam experiment groups （groups L60- 300）, lornoxicam 60, 120, 180, 240, and 300 μg were administered intrathecally, respectively, and followed by rat intra-plantar administration of 100 μL of 5% formalin. The graded pain behavior was observed and the nociceptive behavior index （NBI） was calculated per 5 rain during one hour immediately after the intra-plantar injection. At the 2-hour time point, all the rats were deeply anesthetized and perfused intracardially; and then, the spinal cord was removed for analysis of c-Fos protein-like immunoreactive neurons （FLIN） at the dorsal horn in the L4-L6 segments. The Student-Newman- Keuls test was used to identify the differences between groups,and correlation analysis was used to demonstrate the relationship between rat behavior and c-Fos expression. Results （1） Almost no pain response was observed in group NS. The typical biphasic pain response was observed in the formalin-injected groups. In phase Ⅰ , no difference in the NBI was found between the formalin-injected groups except group L240 and group L300 in which NBI were significantly smaller than that in other formalin-injected groups. In phase Ⅱ , the cumulative NBI in phase Ⅱ decreased with the increase in lornoxicam dosage, but the difference was not significant when the

关 键 词：鞘内注射 氯诺昔康 福尔马林模型 行为学 C-FOS 

分 类 号：R96[医药卫生—药理学]