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机构地区:[1]四川大学华西医院烧伤整形科,成都610041
出 处:《四川大学学报(医学版)》2008年第1期111-113,共3页Journal of Sichuan University(Medical Sciences)
摘 要:目的探讨皮下局部注射重组人结缔组织生长因子(rCTGF)对超长比例皮瓣血管生成的影响。方法在大鼠背部建成8cm×2cm的超长全厚随意皮瓣。术后在皮瓣分别距蒂端4cm、7cm的中线处皮下注射rCTGF(实验组,rCFGT120ng、160ng、200ng)或生理盐水(对照组)。注射完毕,立即原位缝合皮瓣。术后10d将皮瓣平均分成近、中、远三段,选取分别距蒂部1.5cm、4.5cm和7cm的皮瓣组织块进行石蜡包埋,切片和HE染色。并用因子对皮瓣的近,中两段组织中的血管内皮细胞进行免疫组化染色,对血管数进行计数。结果对照组的近段皮瓣血管数为13.88±1.959,rCRGF小、中、大剂量实验组皮瓣近段血管数分别为21.00±3.423,27.38±3.114,37.38±3.583。实验组与对照组之间的差异均有统计学意义(P<0.05)。对照、rCRGF小、中、大剂量组皮瓣中段血管数分别为9.50±1.927,15.50±2.777,24.63±3.335和28.63±4.984,各组之间的差异有统计学意义(P<0.05)。结论局部注射rCTGF能诱导皮瓣新生血管形成,从而促进皮瓣的存活,且该作用呈剂量依赖性。Objective To investigate the effects of connective tissue growth factor (CTGF) on angiogenensis of random skin flaps in rats. Methods Thirty-two SD rats were randomLy divided into three CTGF groups (rCTGF 120 ng, rCTGF 160 ng, rCTGF 200 ng) and one control group. Ischemic random dorsal skin flaps (2 cm× 8 cm) were constructed on the backs of the rats. In the CTGF groups, connective tissue growth factor (CTGF) was injected subcutaneously to the rats in the skin flaps at the time of surgery. The same volume of 0. 9% NaCl solution was injected to the rats in the control group. Ten days later, the amount of viable tissues within the flaps were examined by planimetry. Specimens from the random skin flaps were harvested for immunohistological and HE staining to examine the microvascular growth. Results The immunohistochemical staining and HE staining revealed that the three CTGF groups had more vessels than the control group (21.00± 3. 423,27. 38± 3. 114 and 37.38± 3. 583 vs 13.88± 1. 959,P〈0. 05). Similar results were found in the middle parts of the skin flaps (15.50±2. 777,24.63±3. 335 and 28.63±4. 984 vs 9.50±1. 927, P〈0. 05). Conclusion CTGF may enhance the survival of skin flaps in a dose-dependent manner, which is associated with an increase in angiogenesis of skin flaps.
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