多房棘球绦虫重组BCG-Em14-3-3疫苗不同接种途径诱导小鼠细胞因子的研究  

Cytokines of Splenocytes in Mice by Different Vaccination with Recombinant BCG-Em14-3-3 Vaccines Against Echinococcus Multilocularis

作　　者：李文桂[1] 王鸿[1] 朱佑明[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《四川大学学报（医学版）》2008年第1期130-132,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30671835;30500423和30200239)

摘　　要：目的探讨多房棘球绦虫重组BCG-Em14-3-3疫苗免疫和Em原头节攻击后小鼠脾细胞因子的变化。方法将疫苗通过皮下注射和鼻腔内接种分别免疫BALB/c鼠,免疫后8周用多房棘球绦虫原头节进行攻击感染,感染后18周杀鼠取脾,分离脾细胞,用EmAg或ConA刺激培养,收集脾细胞培养上清液,用试剂盒检测脾细胞培养上清液的IL-2、IFN-γ、TNF-α和IL-4水平,同时设有PBS对照。结果疫苗接种组的IFN-γ和TNF-α水平升高,IL-4水平降低;鼻腔内接种组的TNF-α水平高于皮下注射组。结论多房棘球绦虫重组BCG-Em14-3-3疫苗诱导小鼠产生一个TH1型反应,从而对抗Em原头节攻击感染;疫苗鼻腔内接种是一种较好的免疫途径。Objective To detect the changes of eytokines of splenoeytes in mice immunized by recombinant BCG-Em14-3-3 vaccines against Echinococcus rnultilocularis（Em）. Methods BALB/c mice were subcutaneously and intranasally vaccinated by the BCG-Em14-3-3 vaccines respectively, with PBS as a control. The mice were challenged with Em protoseoleees eight weeks after the vaccinations. The spleens of the mice were collected 18 weeks after the infections. The splenoeytes were separated and cultured with the stimulation of EmAg or ConA. The IL-2,IFN-γ, TNF-α and IL-4 in the supernatants were measured with ELISA. Results Significant increase of IFN-γ and TNF-α were found in the immunized mice. But the IL-4 was decreased after the vaccinations. The levels of TNF-α were higher in the intranasal vaccinated mice than in the subcutaneous vaccinated mice. Conclusion TH1 response is induced in mice by rBCG-Em14-3-3 vaccines to fight against the challenge of Em protoseoleees. Intranasal vaccinations are preferable.

关 键 词：多房棘球绦虫 重组BCG—Em14—3—3 疫苗 细胞因子 

分 类 号：R392[医药卫生—免疫学]