高浓度吸氧对大鼠脑组织抗氧化能力的影响  

EFFECTS OF HIGH-CONCENTRATION OXYGENATION ON RAT CEREBRAL ANTIOXIDANT ABILITY

作　　者：郭长春[1] 袁静[1] 李涵葳[1] 刁文波[1] 任永功[1] 

机构地区：[1]暨南大学第二临床医学院,广东深圳518020

出　　处：《现代医院》2008年第1期2-4,共3页Modern Hospitals

摘　　要：目的探讨吸氧对脑细胞抗氧化损伤能力的影响及其机理。方法将49只成年雄性Wistar大鼠(230～280g)随机分为7组,组①为正常对照组(不吸氧气);组②、③、④分别吸50%氧气15min、30min、60min;组⑤、⑥、⑦分别吸100%氧气15min、30min、60min。应用Western印迹技术、免疫组化技术和黄嘌呤氧化酶法检测在不同浓度(50%和100%)、不同时间(15、30和60min)吸氧后大鼠脑皮质内NF-κB、iNOS和Mn-SOD的表达变化,同时用病理组织学方法观察脑组织损伤程度。结果与对照组相比,各吸氧组NF-кB均减少;iNOS在50%吸氧15min、30min组和100%吸氧15min组阳性细胞数显著增加,其余各组阳性细胞数虽有增加,但差异无显著意义;各吸氧组Mn-SOD活力均增加,但仅100%吸氧60min组有显著意义。病理结果为:50%吸氧各组毛细血管充血、出血,神经元轻度水肿;100%吸氧各组神经元呈重度水肿,核嗜酸样变性,有噬神经现象,病理改变程度与吸氧时间无关。结论50%浓度吸氧可引起轻度可逆性脑损伤,100%浓度吸氧可引起严重的不可逆性脑损伤。吸氧不能活化NF-κB,反而使其表达下降。Objective To investigate the effects and mechanisms of oxygenation on rat cerebral antioxidant ability. Methods 49 adult male Wistar rats weighting 230 -280 g were randomly divided into seven equal groups with seven animals each. 1 group was normal controls; 2,3,4 group was exposed to oxygenation （50% 02 ） for 15,30 ,60min; 5 -7 group was exposed to oxygenation （100% 02 ） for 15,30,60 min. We examined the expression of Nu- clear FactorKB, induce Nitric Oxide Synthase and Mn - Superoxide Dismutase at protein level in the brain of rat by Western blotting analysis, immunohistochemistry and xanthine oxidase analysis. All the brains of rats were examination using light microscope to determine the degree of neuronal damage. Results The expression of NF - KB all decreased in oxygenation groups compared with that in the control group; The numbers of iNOS positive cells all increased as compared with that in the control group. But that only significantly increased in oxygenation （ 50% 02 ） for 15,30 min and oxygenation （ 100% O2 ） for 15min groups. The activity of Mn - SOD all increased but that only oxygenation （ 100% O2 ） for 60min group increased significantly. Pathology results ： The harm of neuzons in oxygenation （ 100% 02 ） groups was more serious than in oxygenation （ 50% 02 ） groups. The neurons were severe edema and eosinophilic degeneration and so on. Pathology change grade had not relations with oxygenation times. Conclusion 50% oxygenation can lead to slightly reversible injury, but 100% oxygenation can lead to serious unreversible injury. Oxygenation can not activate NF - KB, those expression all decreased on the contrary.

关 键 词：吸氧 核因子KB INOS MN-SOD 

分 类 号：R644[医药卫生—外科学]