大孔吸附树脂富集纯化大黄总蒽醌的工艺研究  

Study on Purification Process of Total Anthraquinones in Radix et Rhizoma Rhei with Macroreticular Resin

作　　者：徐晶[1] 陈再兴[2] 袁昌鲁[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]中国医科大学,辽宁沈阳110001

出　　处：《中华中医药学刊》2008年第1期115-116,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省教育厅立项研究课题(200402270)

摘　　要：目的:筛选纯化大黄总蒽醌的最佳树脂,确定树脂纯化大黄总蒽醌的工艺参数。方法:以大黄中总蒽醌含量为指标,研究大孔吸附树脂富集、纯化大黄总蒽醌的吸附性能和洗脱参数。结果:X-5型树脂对大黄总蒽醌有良好的吸附性能。洗脱剂为60%乙醇,用量为6倍量树脂柱体积,大黄总蒽醌富集于60%乙醇洗脱液部分,且除杂质效果好。结论:通过X-5型大孔吸附树脂富集、纯化后,大黄总蒽醌的洗脱率在90%以上,总蒽醌的含量提高到35.4%。说明采用本法富集、纯化大黄总蒽醌是可行的。Objective ： To optimize the macroretieular resin, to establish the technological parameters of the purification process of total anthraquinones in Radix et Rhizoma Rhei with macroreticular resin. Methods： The adsorptive characteristies and elutive parameters of the process were studied by taking the content of total anthraquinones as marker. Results ： X - 5 type macroreticular resin has good purification process, the extractive of total anthraquinones was washed with distilled water, then was washed with 6 - fold times 60% alcohol for macroreticular resin. The total anthraquinones in Radix et Rhisoma Rhei was eluted with 60% alcohol. Conclusion： With X -5 type macroreticular resin to adsorb and purify,the elutive ratio of total anthraquinones in Radix et Rhizoma Rhei was over 90% and the purity reached 35.4%. So this process of applying macroreticular resin to adsorb and purify total anthraquinones in Radix et Rhizoma Rhei is feasible.

关 键 词：大孔吸附树脂 大黄总蒽醌 纯化工艺 

分 类 号：R284.2[医药卫生—中药学]