不同宿主源禽腺病毒分离株对雏鸡的致病性比较  

Comparison of Pathogenicity between Fowl adenovirus from Different Hosts

作　　者：孙锦[1] 黄兵[2] 李珍祖[1] 杨军[3] 王可洲[4] 李云龙[1] 

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]山东省农业科学院家禽研究所,济南250023 [3]山东中医药大学第二附属医院,济南250100 [4]山东省医学科学院实验动物中心,济南250002

出　　处：《家禽科学》2008年第1期4-6,共3页Poultry Science

基　　金：山东省农业科学院博士基金资助(No.2006YBS018)

摘　　要：鸭腺病毒1型病毒可作为优良的重组疫苗载体。为了筛选低毒力的鸭腺病毒1型病毒毒株,分别用病毒QU株和HS株对20日龄SPF鸡进行攻毒,4d后检测其血清生化指标的变化情况并与对照组进行比较,同时取其肝脏进行病理组织学观察。发现QU组的各项生化指标均没有明显变化,肝组织形态与对照组相同;HS组的谷草转氨酶、碱性磷酸酶、胆碱脂酶和血淀粉酶显著降低,总蛋白、白蛋白和球蛋白极显著降低,并且肝细胞大量坏死。结果表明,HS株病毒主要引起鸡肝脏和胰腺的损伤,具有较强的致病性;QU株病毒对雏鸡的生理状况几乎没有影响,毒力很弱,适合作为重组疫苗载体。Duck adenovirus type 1 will be excellent vector of gene vaccine. In order to get an avirulent DAV1 strain, chickens at the age of 20 days were infected with either QU strain or HS strain. Four days post challenge several serum biochemical indices and hepatic tissue sections were determined. In contrast to healthy chickens, serum biochemical indices and hepatic tissue sections of chickens infected with QU strain displayed no obvious changes. However in the group that received HS strain, a sharp decline of the levels of serum TP, ALB, GLOB, AST, ALP, CHE and AMY was noticed, and necrosis of hepatocytes was detectable by optical microscope.These findings suggest that HS strain mainly leads to losses in liver and pancreas, while QU strain as potential engineering vector can hardly affect the physiology of chickens.

关 键 词：鸭腺病毒1型 血清生化指标 病理组织学 毒力 

分 类 号：S858.31[农业科学—临床兽医学]