瓦氏黄颡鱼仔稚鱼发育过程中消化酶活性变化研究  

Assessment of digestive enzymes activities during larval development of Pelteobagrus vachelli

作　　者：李芹[1] 龙勇[2] 屈波[1] 罗莉[1] 刁晓明[1] 

机构地区：[1]西南大学动物科技学院水产科学系,重庆400715 [2]河南信阳农业高等专科学校水产系,河南信阳464000

出　　处：《中国水产科学》2008年第1期73-78,共6页Journal of Fishery Sciences of China

摘　　要：测定了瓦氏黄颡鱼(Pelteobagrus vachelli)仔稚鱼从出膜至30日龄时的生长、可溶性蛋白含量及消化酶活性。结果表明,在仔鱼出膜后第1天,蛋白酶、淀粉酶、脂肪酶均能检测到活性,而胃蛋白酶活性在第22日龄才检测到。可溶性蛋白的含量随日龄的增加而增加。蛋白酶、淀粉酶、脂肪酶的活性随着仔稚鱼的发育而发生变化,其全活力与比活力呈现出不同的变化模式。蛋白酶比活力在仔鱼4日龄时达到最大值(4.193±0.04)U/mgprotein;之后随日龄的增加比活力降低,直至15日龄达到最小值(0.452±0.07)U/mgprotein。之后从15日龄到30日龄比活力逐渐增加。淀粉酶比活力从1日龄逐渐增加,至4日龄达到最大值(4.611±0.12)U/mgprotein,从10日龄到18日龄维持在一较低水平,随后随日龄的增加淀粉酶比活力增加。脂肪酶比活力从1日龄到3日龄逐渐增加,到3日龄达到最大值(4.398±0.07)U/mgprotein,3日龄以后直到30日龄,脂肪酶比活力随日龄的增加而降低,并且一直处于一较低的水平。瓦氏黄颡鱼发育过程中,主要消化酶活性随生长变化显著,反映瓦氏黄颡鱼随着生长其消化功能逐渐完善。Growth, soluble protein content and the activities of some digestive enzymes were studied in Pelteobagrus vachelli larvae during the first month after hatchery. The results of experiment were as follows： on the first day, the activities of protease, amylase and lipase were detectable. The activities of pepsin were detectable on the 22nd day. Soluble protein content increased with the increase of age. The activities of digestive enzymes presented a series of changes with the increase of age. The variation model was different between total activities and specific activities. From day 1 to day 4, the protease activities increased dramatically to the maximum value of （4.193 ± 0.04）U/mg protein. Then, the protease specific activities decreased progressively with the increase of age. On the 15th day, the specific activities reached the minimum point of （0.452 ± 0.07） U/ mg protein. From day 15 to day 30, the protease activities increased progressively with the increase of age. From day 1 to day 4, amylase activities reached the maximum point of （4.611±0.12）U/mg protein, then decreased sharply to very low levels on 10th day. From day 10 to day 18, the amylase specific activities maintained at the same levels. After day 18, the activities increased with the increase of age. From dayl to day 3, the lipase specific activities increased sharply to the peak of （4.398±0.07） U/mg protein on the 3rd day, then followed by a decrease to very low levels, which were maintained from day 3 to day 30. Important variations in activities of all the digestive enzymes were observed during larval development of Pelteobagrus vachelli. The conclusion is that the activities of the main digestive enzymes in P.vachelli changes obviously, implying the digestive function will be completed progressively with its development. [Journal of Fishery Sciences of China, 2008, 15 （1） ： 73-78]

关 键 词：瓦氏黄颡鱼 发育 消化酶 酶活性 

分 类 号：Q959.499[生物学—动物学]