感染桃拉综合症病毒的凡纳滨对虾体内4种同工酶酶谱的变化  

Changes of four isozymes in different tissues of Litopenaeus vannamei with Taura Syndrome Virus

作　　者：孙静秋[1] 许燕[1] 李晓英[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《中国水产科学》2008年第1期122-128,共7页Journal of Fishery Sciences of China

基　　金：上海市教委高校科技发展基金项目(No.05DZ30).

摘　　要：采用聚丙烯酰胺凝胶垂直平板电泳法,对凡纳滨对虾(Litopenaeus vannamei)成体的复眼、肝脏、心脏、鳃和肌肉等5种组织中的酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、酯酶(EST)和过氧化物酶(POD)4种同工酶进行研究,并将健康虾与感染桃拉综合症病毒(TSV)病虾的同工酶酶谱进行比较。结果表明,这4种同工酶在凡纳滨对虾体内的不同组织均有分布,但在酶活性和条带数量上存在不同程度的组织特异性,其中4种同工酶均在肝脏组织中表达最强,ACP和POD在肌肉中、EST在复眼中表达最弱,AKP在复眼中不表达。感染TSV后,病虾的ACP和AKP均出现酶带数量减少和酶活性降低,如病虾的ACP酶谱在肝脏、心脏和鳃中缺失的酶带数分别为2、1和1条,酶活性也呈现下降;AKP酶谱在肝脏、心脏和肌肉中缺失的酶带数分别为3、4和3条,且酶活性均明显降低;而病虾的EST和POD表达的酶带数和酶活性却均有明显增加,尤其是在肝脏和心脏中,EST分别新增3条和5条酶带,POD各新增1条酶带。总之这4种同工酶的表型在健康虾和病虾之间存在着明显的差异,尤其是在肝脏中的变化最为明显,说明肝脏中这4种同工酶的特异性变化可以作为研究凡纳滨对虾桃拉综合症的生理生化指标。Four isozymes including phosphatase （ACP）, alkaline phosphatase （AKP）, esterase （EST） and peroxidase （POD） in eye, heart, liver, gill and muscle of Litopenaeus vannamei were ananlysed and compared between the health shrimp and the diseased shrimp infected by Taura Syndrome Virus （TSV） through vertical polyacrylamide gel electrophoresis （PAGE）.The results showed that the four isozymes were all expressed in five kind of tissues of L.vannamei, but the band numbers and activities of the four isozymes were tissue specific to some extent.The activities of the four isozymes were all the highest in liver of L.vannamei, while those of POD and ACP were the lowest in muscle; EST activity was the lowest in eye, and no AKP activity was expressed in eye. There were evident differences between diseased shrimp and healthy shrimp in zymograms and activities of ACP, AKP, EST and POD in tissues. The ACP and AKP activities decreased after infection with TSV. ACP decreased by 2, 1 and 1 band in liver, gill and heart, respectively and some bands got weaker; AKP decreased by 3, 4 and 3 bands in liver, heart and muscle respectively.But higher activities and more complex zymograms of EST and POD were detected in diseased shrimps than those in the healthy shrimps especially in liver and heart. EST presented 3 new bands and 5 new bands, and POD presented 1 new band in liver and heart of the disease shrimp respectively, furthermore some bands got deeper and thicker.Due to the electrophoretogram of four isozymes changed most obviously in liver of diseased shrimps with TSV, the isozymes in shrimp liver can be used as biochemical marker for diagnosis of Taura Syndrome in L.vannamei. [Journal of Fishery Sciences of China, 2008, 15 （1）： 122-128]

关 键 词：凡纳滨对虾 桃拉综合症病毒 同工酶 聚丙烯酰胺凝胶电泳 组织特异性 

分 类 号：Q959.223[生物学—动物学]