固定化氨基酰化酶载体结构及在拆分中的应用  

Structure of Immobilized Aminoacylase and Its Application in Resolution

作　　者：杨瑞东[1] 赵林[1] 宋正孝[1] 郭红彦[1] 

机构地区：[1]天津大学化工学院,天津300072

出　　处：《化学工业与工程》2008年第1期5-8,共4页Chemical Industry and Engineering

基　　金：天津市自然科学基金资助项目(043802611)

摘　　要：对采用大孔弱碱性阴离子树脂DEAE-D/H为载体制备得到的高活性固定化氨基酰化酶的载体的结构进行了扫描电镜分析,具有这种结构的DEAE-D/H固定化酶活力接近1 400 U/g。采用自制的固定床反应器连续拆分乙酰DL-蛋氨酸,根据对停留时间的测量,在反应器的液体流动为全混流、液体流速低于25 mL/h时,转化率接近100%。固定化酶具有良好的连续操作稳定性和贮藏稳定性。The structure of macroporous weakly basic anion resin（DEAE-D/H） with immobilized aminoacylase was analyzed by environmental scanning electron microscope. The result showed the specific activity of DEAE- D/H with immobilized aminoacylase was up to 1 400 U/g. The retention time was measured in packed bed reactor for the resolution of N-acety-methionie, and the liquid flow was continuous stirred. The conversion of L-methionine at the volumetric flow rate below 25 mL/h was nearly 100%. The immobilized aminoacylase was prepared with high stability of continuous operation and storage.

关 键 词：固定化 结构研究 氨基酰化酶 固定床反应器 

分 类 号：TQ426.97[化学工程]