可乐对小鼠精子质量影响  

Effect of cola on quality of spermatozoa of mice

作　　者：刘舒瑜[1] 李芝兰[1] 骆晓红[1] 赵小莉[1] 王进科 蒲育栋[1] 党瑜慧[1] 安婧[1] 

机构地区：[1]兰州大学公共卫生学院,兰州730000

出　　处：《中国公共卫生》2008年第1期77-79,共3页Chinese Journal of Public Health

基　　金：2006年兰州大学学生创新创业行动项目(LZDX-JT200602-178)

摘　　要：目的探讨可乐对雄性小鼠精子质量及数量的影响。方法31只昆明种雄性小鼠按体重随机分为阴性对照组、低剂量组、高剂量组(分别以0.6 ml生理盐水、0.3,0.6 ml可乐灌胃),腹腔注射组(0.6 ml可乐注射)和阳性对照组(0.6 ml环磷酰胺腹腔注射)5组。灌胃及腹腔注射1次/d,连续7 d,测定小鼠精子的运动参数,并观察其畸形发生率。结果高剂量组肾脏系数与阴性对照、低剂量组比较差异均有统计学意义(P<0.05);腹腔注射组与阴性对照组比较小鼠附睾系数明显升高(P<0.05)。腹腔注射组的精子平均移动角度与阴性对照组比较明显升高(P<0.05),低剂量组精子密度与阳性对照组比较差异有统计学意义(P<0.05)。高剂量组、阳性对照组与阴性对照组比较精子畸形率明显升高(P<0.05);低、高剂量组及腹腔注射组与阳性对照组比较差异均有统计学意义(P<0.05);低、高剂量组与腹腔注射组比较差异均有统计学意义(P<0.05)。结论可乐对小鼠精子活性和形态有一定的影响。Objective To explore the effect of cola on the quality and quantity of spermatozoa of male mice.Methods 31 male mice of Kunming strain were divided randomly into 5 groups：negative control group treated with normal saline 0.6 ml;lowdose group with 0.3 ml of cola,high-dose group with 0.6 ml of cola;abdominal injection group with 0.6 ml of cola and positive control group with 0.6 ml of cytoxan once daily for 7 days.Then,the mice were killed and their epididymis were collected and the movement parameter and deformity of the sperm were observed.Results The kidney coefficient of high-dosage group had the significance difference compared with positive control group and low-dosage group.The epididymis coefficient of abdominal injection group was significantly higher than that of positive control group（P〈0.05）.HB,PLT and WBC of every group was lower than positive control group.MAD of abdominal injection group was significantly higher than positive control group.The ＂ρ＂ of low-dosage group had the significance difference compared with positive control group（P〈0.05）.The sperm deformity rates of low-dosage group,high-dosage group and negative control group were significantly higher than that of positive control group（P〈0.05）.The sperm deformity rates of low-dosage group,high-dosage group and abdominal injection group had the significance difference compared with negative control group（P〈0.05）.The sperm deformity rates of low-dosage group and high-dosage group had the significance difference compared with abdominal injection group（P〈0.05）.Conclusion Cola has certain effect on spermatozoa activeness and its forms of male mice.

关 键 词：可乐 小鼠 精子运动参数 精子畸形 灌胃 

分 类 号：R173[医药卫生—妇幼卫生保健]