硒和抗坏血酸对砷致大鼠细胞毒性拮抗作用  

Antagonistic effects of selenium and vitamin C on cytotoxicity of arsenic to rats

作　　者：高颖[1] 赵莉莎[2] 

机构地区：[1]沈阳医学院药理教研室,沈阳110034 [2]辽宁省职业病防治院,沈阳110034

出　　处：《中国公共卫生》2008年第1期116-117,共2页Chinese Journal of Public Health

摘　　要：目的观察不同浓度的亚砷酸钠(NaAsO2)对体外培养的大鼠淋巴活性氧群(ROS)和细胞内脂质过氧化物(LPO)含量的影响,并探讨亚硒酸钠(NaSeO3)和维生素C对砷毒性的拮抗作用。方法体外分离大鼠外周血淋巴细胞后,施加处理因素,在37℃条件下恒温培养,用2’7’-二乙酰二氯荧光素(DCFH-DA)染色法检测细胞内的ROS水平,用硫代巴比妥酸荧光法测定细胞内LPO含量。结果NaAsO2浓度>10μmol/L时,ROS和乙二醛(MDA)含量明显高于对照组;用抗氧化剂预处理使细胞内ROS和MDA含量减少。结论亚砷酸钠可引起细胞内氧化应激,抗氧化物可一定程度拮抗砷的细胞毒性作用。Objective To investigate the effect of different concentrations of sodium arsenite on the reactive oxygen species（ROS） and lipid peroxidate（LPO） of the lymphocytes of rats cultured in vitro and the antagonism of sodium selenite and vitamin C（Vit.C）.Methods The lymphocytes of rats were isolated from the peripheral blood and cultured with sodium arsenite under 37 ℃.The ROS content was detected by DCFH-DA;The content of LPO was detected by fluoresce method.Results The contents of ROS and MDA increased significantly when the sodium arsenite was above 10μmol/L;After pre-treatment of sodium selenite for 24 hours,the contents of ROS and MDA decreased in sodium arsenite of 10μmol/L.Conclusion Sodium arsenite can induce oxidative stress,and antioxidants can antagonize the cytotoxicity of arsenic.

关 键 词：亚砷酸钠 亚硒酸钠 维生素C 活性氧群 淋巴细胞 

分 类 号：Q538[生物学—生物化学]