SAH抑制内皮细胞增殖和ER-α表达作用  

Effect of S-adenosylhomocysteine on inhibitory proliferation induced by ER-α expression in endothelial cells

作　　者：余小平[1] 刘驰[1] 夏敏[1] 王庆[1] 迟东升[1] 凌文华[1] 

机构地区：[1]中山大学公共卫生学院营养系

出　　处：《中国公共卫生》2008年第1期119-121,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30571568);中国博士后科学基金(2005037174)

摘　　要：目的探讨胞内S-腺苷同型半胱氨酸(SAH)升高抑制血管内皮细胞增殖和雌激素受体-α(ER-α)表达变化的关系。方法以永生化的人脐静脉内皮细胞(HUVEC)为研究对象,经不同浓度的S-腺苷同型半胱氨酸水解酶(SAHH)强效抑制剂3-deazaadenosine(DZA)处理24,48,72 h。利用细胞计数法、流式细胞仪和脱氧核糖核酸转移酶缺口末端标记法(TUNEL)检测细胞的增殖凋亡能力,蛋白印迹法检测ER-α蛋白的表达,荧光定量-PCR(qRT-PCR)检测其mRNA表达,甲基化特异PCR法(MSP)检测ER-α基因启动子甲基化状态。结果经DZA处理后,HU-VEC增殖能力降低(主要受阻于G1/G0期),细胞凋亡率增加(P<0.05),ER-αmRNA和蛋白的相对表达量降低(P<0.05),但其启动子甲基化不发生改变。结论胞内SAH升高抑制HUVEC的增殖能力与ER-α的表达变化有关,但这种作用不是通过改变ER-α基因启动子的甲基化而实现。Objective To explore the relationship of inhibitory proliferative ability induced by intracellular S-adenosylhomocysteine（SAH） accumulation and the expression of estrogen receptor-α（ER-α） in human umbilical vein endothelial cells.Methods Immortalized HUVEC were treated without（normal） or with different concentration 3-deazaadenosine（DZA）,a potent S-adenosylhomocysteine hydrolase（SAHH） inhibitor,for 24,48 and 72h.The proliferative ability of HUVEC was measured with flow cytometry and TdT mediated dUTP nick ending labelling（TUNEL） methods.The ER-α protein and mRNA expression was analyzed with western blot and quantitative reverse transcription-polymerase chain raction（qRT-PCR） respectively.The promoter methylation of ER-α gene was detected with methylation special PCR（MSP）.Results The proliferative ability was decreased（mainly arrested at G1/G0 phase）,and the apoptotic rate was increased after HUVEC treatment with DZA（P〈0.05）.The relative expression of ER-α mRNA and protein in HUVEC treated with DZA was lower than that of normal（P〈0.05）,but there was no changes of ER-α gene promoter methylation.Conclusion The inhibitory proliferative ability induced by intracellular SAH accumulation is correlative with ER-α expression,which not through altering promoter methylation in HUVEC.

关 键 词：S-腺苷同型半胱氨酸 雌激素受体-Α 人脐静脉内皮细胞 甲基化 

分 类 号：R151.41[医药卫生—营养与食品卫生学]