小鼠脑损伤相关基因的克隆及表达与生物学功能初步分析  

Cloning,expression and preliminary functional analysis of a novel gene related to brain injury in mice

作　　者：冯刚[1] 邱俊[1] 杨志焕[1] 朱佩芳[1] 王正国[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所,创伤,烧伤与复合伤国家重点实验室,重庆400042

出　　处：《创伤外科杂志》2008年第1期44-47,共4页Journal of Traumatic Surgery

基　　金：国家重点基础研究发展规划项目(G1999054203);国家自然科学基金项目(303000333)

摘　　要：目的克隆1个与小鼠脑损伤早期反应相关的新基因(GBI),并对其功能进行初步分析。方法SMART-RACE技术克隆该新基因全长cDNA;采用生物信息学软件分析其可能的功能结构域和进行序列相似性分析;Northern Blotting分析该基因在小鼠主要组织器官的生理水平的表达。结果该新基因全长cDNA序列为1798bp,含1个编码249个氨基酸残基蛋白质的开放性阅读框。推导蛋白质分子与1个即刻早期蛋白IE175和1个应激相关蛋白RAB21具有部分序列相似性。Northern杂交显示其仅仅在小鼠脑组织有基础水平的表达。结论该基因可能与创伤早期反应的应激信号转导相关,并命名为脑损伤相关基因GBI(gene related to brain injury,gi|19338981|gb|AF481964.1)。Objective To clone the full length cDNA of a novel gene related to early response to acute brain injury in mice and analyze the function of this gene preliminarily. Methods SMART-RACE strategy was applied to clone the full length cDNA. The putative functional motif and protein sequence alignment were demonstrated with bioinformatics software. The basal expression of this novel gene in tissues and organs of mice were detected with northern blotting. Results A 1798bp cDNA contig was identified. The isolated cDNA contained a 249 amino acids open reading frame. The putative protein had partial sequence similarity to an immediate-early protein IE175 and a stress-associated protein RAB21. Northern blotting analysis indicated that the novel gene had a baseline ex- pression only in brain of mice. Conclusion It is suggested that this novel gene may be involved in the stress signal pathway in the early stage of acute brain injury,and named it GBI（ gene related to brain injury, gi119338981 ｜ gbl AF481964.1 ）.

关 键 词：颅脑损伤 克隆 基因 生物学 

分 类 号：R651.15[医药卫生—外科学]