鸡艾美耳球虫单卵囊分离与种类鉴定  

Single-oocyst Isolation and Species Identification of Eimeria in Chicken

作　　者：王鑫[1] 黄兵[1] 赵其平[1] 韩静芳[1] 韩红玉[1] 董辉[1] 姜连连[1] 

机构地区：[1]中国农业科学院上海兽医研究所农业部动物寄生虫学重点开放实验室,上海200232

出　　处：《复旦学报（自然科学版）》2007年第6期930-936,共7页Journal of Fudan University：Natural Science

基　　金：国家科技基础条件平台建设项目(2005DKA21104)

摘　　要：为了建立鸡球虫纯株,选用1-5日龄雏鸡,采用琼脂块单卵囊分离法对实验室保存的6种11株鸡球虫进行纯化,并选用2周龄雏鸡,对每个虫种/株选取1个单卵囊分离物进行增殖和种类鉴定.结果单卵囊接种179只,获得单卵囊分离物42个,单卵囊分离成功率23.46%;对11个单卵囊分离物,通过测定寄生部位、潜在期、孢子化卵囊大小、形状指数（长/宽）、孢子囊大小、最短孢子化时间等指标,鉴定出1个分离物为堆形艾美耳球虫（Eimeria acervulina）、2个分离物为柔嫩艾美耳球虫（E.tenella）、6个分离物为巨型艾美耳球虫（E.maxima）、1个分离物为变位艾美耳球虫（E.mivati）、1个分离物为毒害艾美耳球虫（E.necatrix）.本研究表明琼脂块单卵囊分离法简便易行、成功率较高,同时提示实验室保存的布氏艾美耳球虫（E.brunetti）被柔嫩艾美耳球虫污染.To identity the strains of Eimeria conserved in our laboratory are purebred, 11 strains of 6 Eimeria species were separated by single-occyst method and infected chicken of 1-5 days old. 179 chicken were inoculated and 42 of them were infected successfully. The rate of successful is 23.46 %. Then the 11 of 42 isolated-strains were identified according to the location of coccidia in the intestine, prepatent period, size and shape of occysts, shape index （ length divided width）, size and shape of sporocysts, the shortest sporulation time. 1 strain of E. acervulina ,2 of E. tenella ,6 of E. maxima, 1 of E. mivati and 1 of E. neratrix are identified, respectively. These results indicated that the E. brunetti Guangdong strain conserved in our laboratory was polluted by E. tenella.

关 键 词：鸡 球虫 艾美耳球虫 单卵囊 

分 类 号：S858.312.723[农业科学—临床兽医学]