三疣梭子蟹第一次卵巢发育过程中卵母细胞和卵泡细胞超微结构的观察  

Ultrastructure of Oocytes and Follicular Cells of Portunus trituberculatus during the First Reproductive Cycle

作　　者：杨筱珍[1] 吴旭干[1] 姚桂桂[1] 成永旭[1] 王春琳[2] 

机构地区：[1]上海水产大学省部共建水产种质资源发掘与利用教育部重点实验室,上海200090 [2]宁波大学生命科学与生物工程学院,宁波315211

出　　处：《复旦学报（自然科学版）》2007年第6期963-967,共5页Journal of Fudan University：Natural Science

基　　金：国家高技术研究发展计划资助项目(2006AA10-A406);浙江省重大科技攻关项目(2004C12029);农业部水产种质资源和养殖生态重点开放实验室开放课题(KFI2006-3);上海市重点学科建设项目(Y1101)

摘　　要：通过透射电镜技术研究了东海三疣梭子蟹初产卵巢发育过程中卵巢的超微结构的变化.1）三疣梭子蟹初产卵巢发育过程中卵黄发生期可分为初期和后期;2）卵黄发生初期在7-9月间,此阶段卵黄生成以内源性合成为主,卵母细胞核物质外输明显,胞浆内大量的粗面内质网正在进行卵黄物质的内源性合成;3）卵黄发生后期在10月至第二年4月间,卵泡细胞与卵母细胞的结合阶段,卵母细胞借助卵泡细胞进行外源性卵黄物质合成,卵母细胞与卵泡细胞间的界线逐渐消失,部分卵泡细胞由于胞质大量输出仅见胞核,此时内源性卵黄合成仍然存在;4）排卵后,由于大量成熟卵细胞的排出,卵巢组织存在大量的基膜,残留成熟卵母细胞中的卵黄物质已经解体,卵黄颗粒自中央向边缘逐渐松散.The study was conducted to investigate the ultrastructure of Portunus trituberculatus ovary during the first ovary development by transmission microscopy. The results showed that： （1） Vitellogenesis in the Portunus trituberculatus can be divided in two stages： primary and secondary stages, （2） In primary stage （in Jul-Sep）, vitelline was formed mainly by intro-oocytic yolk formation. A few of nucleolus-liked substance released from nucleolus of oocytes. Many vesides of the endoplamic reticulum with vitellogenesis in ooplasm were observed. （3） In secondary stage （in Oct-next year Apr）, many follicular cells have combined with oocytes, then the substances of yolk from extro-oocytic were transported into ootytes by follicular cells. Meanwhile, intro-oocytic yolk accumulation still existed. Many rough endoplamic reticulumes with vitellogenesis were found in ooplasm. （4）Many basal membranes were observed easily after mature oocytes releasing from ovary. The yolk substance of residual mature oocytes disaggregated, the outside substance of yolk distributed loosely in degenerate oocytes.

关 键 词：三疣梭子蟹 卵黄发生 卵母细胞 卵泡细胞 超微结构 

分 类 号：Q248[生物学—细胞生物学] S917.4[农业科学—水产科学]