大批量测定植物(互花米草)叶面积的一种新方法  

A new method of instantly measuring leaf area in large batches: a case study of Spartina alterniflora

作　　者：赵聪蛟[1] 邓自发[1] 周长芳[1] 陈琳[1] 刘静娜[1] 安树青[1] 申瑞玲[1] 

机构地区：[1]南京大学生命科学学院,湿地生态研究所,南京210093

出　　处：《生态科学》2007年第4期327-331,共5页Ecological Science

基　　金：国家自然科学基金资助项目(30400054)

摘　　要：叶面积的准确测定是评价生态系统能流、碳流和水分利用的重要基础。目前常用的叶面积测定方法都有其局限性,有些方法快捷、准确,但仅适用于小批量测定;大多数方法很难在较短时间内准确进行大批量测定。本文以互花米草叶面积测定为例,探讨了以扫描仪和叶片干重为基础,结合PHOTOSHOP图像处理技术,大批量、快捷、准确测定植物叶面积的方法——扫描-干重法。结果表明:虚拟正方形叶片边长与叶子像素成幂函数曲线关系,公式为y=419.85x1.9693,R≈1.00;用此公式验证扫描-干重法,相对误差仅为3.651%。用扫描-干重法测得的结果与方格法相比无显著差异(P=0.473),表明该方法是可行的。Accurate measurement of leaf area is a foundation for evaluating energy flux, carbon flux, and water using of ecosystem. Current methods for measuring leaf area have various limitations and scope of use. Some of them are easy-to-use and accurate, but can only be used for small batches. It is very difficult for most methods to measure leaf areas in large batches in a relatively short period of time. In this study, we took Spartina alterniflora as an example to discuss a method based on scanner and dry-leaves weight, combined with Photoshop image processing technology, which can measure leaf areas instantly in large batches. Tested by a known power function relation between leaf length （y） and leaf pixel （x）, ie. y = 419.85χ^1.9693 （R≈1.00）, our new method only yielded 3.651% （n = 30） relative error. There were no significant difference between this method and a classic square method （P = 0.473）. It is concluded that this method can be used to measure leaf area in large batches in a relatively short period of time, and to measure various shapes of leaves with little change in the vein.

关 键 词：扫描仪 互花米草 叶面积 像素 叶面积曲线 

分 类 号：TP334.22[自动化与计算机技术—计算机系统结构]