采用酵母双杂交法筛选HbMyb1相互作用蛋白  

Screening of the Protein Interaction with HbMyb1 Using Yeast Two-hybrid System

作　　者：邓治[1] 张云霞[2] 陈春柳[1] 陈守才[1] 

机构地区：[1]中国热带农业科学院橡胶研究所农业部热带作物栽培生理学重点实验室,海南儋州571737 [2]海南医学院生物化学教研室,海口571101

出　　处：《植物生理学通讯》2007年第6期1031-1034,共4页Plant Physiology Communications

基　　金：中国热带农业科学院科技基金(Rky050103)

摘　　要：HbMyb1是橡胶树死皮病相关基因,为了进一步研究HbMyb1的功能,采用酵母双杂交方法,从胶乳cDNA文库中筛选出10个与HbMyb1发生相互作用的蛋白,包括已知的橡胶延伸因子和橡胶小粒子蛋白等,其中4个分别与3-羟酰基-ACP-脱氢酶、NAD-依赖的山梨糖醇脱氢酶、氧化还原酶、假定的富含亮氨酸重复蛋白具有同源性的蛋白,其余4个为未知蛋白。HbMyb1 was related to TPD （tapping panel dryness） of Hevea brasiliensis. To study the function of HbMyb1 protein, we used yeast two-hybrid assay to screen a latex cDNA library with full-length of HbMyb1. Ten proteins interacted with HbMyb1 protein were obtained. Among them, 6 proteins with known functions were obtained, including the rubber elongation factor, small rubber particle protein, 3-hydroxyacyl-acyl-carrier protein dehydratase, NAD-dependent sorbitol dehydrogenase enzyme, oxidoreductase and putative leucine-rich repeat protein. 4 proteins with unknown function were found. These work could help for the further study on the function of HbMyb1 and the clarification of mechanism of the TPD.

关 键 词：巴西橡胶树 死皮病 HbMyb1 酵母双杂交 

分 类 号：S763.7[农业科学—森林保护学]