花粉离体培养基的优化和苯达松除草剂对敏感型水稻恢复系‘Mc526’花粉活力的影响  

The Optimization of Pollen Media in vitro and the Influence of Bentazon on Pollen Viability of Rice (Oryza sativa L.) Restorer Line ‘Mc526’

作　　者：王云生[1,2] 谢震[1] 陆徐忠[2] 宋丰顺[2] 李莉[2] 杨剑波[2] 

机构地区：[1]安徽农业大学生命科学学院 [2]安徽省农业科学院水稻研究所,合肥230031

出　　处：《植物生理学通讯》2007年第6期1072-1076,共5页Plant Physiology Communications

基　　金：安徽省科技攻关(重大)项目(06003010B);安徽省教育厅自然科学研究项目(2005KJ169)

摘　　要：建立了适合水稻花粉离体萌发的液体培养基配方,并用花粉萌发和碘.碘化钾(I_2-KI)染色测定法检测了喷施苯达松后含敏感致死基因的水稻恢复系‘Mc526’的花粉生活力。结果表明,苯达松处理前期(施后1～4d),‘Mc526’花粉活力与未作处理的接近;处理后期(施后5～8d)略低于未作处理的,但差异不显著。苯达松对母本结实率影响不显著。A solution medium for pollen germination of rice （Oryza sativa） in vitro was established. After bentazon treatment, the pollen viability of rice restorer line ＇Mc526＇, which included bentazon sensitive lethal （bsl） gene, was researched by using pollen germination and I2-KI pigmentation. The results showed that in the early treated period （1-4 d）, the pollen viability of ＇Mc526＇ was similar to the control. And in the later period （5- 8 d）, the pollen viability was a bit lower than the control, but the difference was not obvious. It indicated that bentazon had no salient influence on the setting rate of sterile line.

关 键 词：水稻 恢复系 苯达松敏感致死基因 花粉活力 液体培养基 

分 类 号：S451.21[农业科学—植物保护]