非洲菊未授粉胚珠的离体诱导和植株再生  

In vitro Induction and Plantlet Regeneration from Unpollinated Ovules of Gerbera jamesonii Bolus

作　　者：王丽花[1] 瞿素萍[1] 杨秀梅[1] 熊丽[2] 王继华[2] 

机构地区：[1]云南省农业科学院农业部花卉产品质量监督检验测试中心,昆明650205 [2]云南省农业科学院花卉研究所,昆明650205

出　　处：《植物生理学通讯》2007年第6期1089-1092,共4页Plant Physiology Communications

基　　金：云南省科技攻关计划(2006NG14)

摘　　要：通过不同培养基及培养条件的筛选,离体诱导12个品种的非洲菊未授粉胚珠的结果表明:MS中的大量元素+ Heller中的微量元素+1/2铁盐+0.2mg·L^(-1)6-BA+0.1mg·L^(-1)IAA较适宜于非洲菊未授粉胚珠愈伤组织的诱导和芽的再生,8个品种中以品种‘E19’诱导愈伤组织的诱导率最高(为23.1%);5个品种可再生形成不定芽,再生率为4.8%-19.6%。用根尖染色体鉴定法鉴定再生的23个植株的倍性的结果显示,21.7%为二倍体,43.5%为单倍体,34.8%为混倍体.By screening of culture media and conditions, the unpollinated ovules in vitro inductions of 12 cultivars of Gerbera jamesonii were studied. The more suitable culture medium for callus and buds induction was consisted of the macronutrients and vitamins of MS, Heller micronutrients, 1/2 Fe-EDTA, 200 mg·L^-16-BA and 100 mg·L^-1IAA. Among 12 cultivars, the callus from 8 cultivars were successfully inducted. Cultivar ＇E19＇ had highest rate of callus-forming （23.1%）; five cultivars regenerated shoots （4.8%-19.6%）. Ploidy was identified by chromosome counting in root tips. The results showed that in those regenerate plantlets diploid was 21.7%, haploid was 43.5%, and mixoploid was 34.8%.

关 键 词：非洲菊 未授粉胚珠 离体诱导 

分 类 号：Q943.1[生物学—植物学]