大花蕙兰高频再生体系的研究  

Study on High-Frequency Regeneration Systerm of Cymbidium hybridium

作　　者：张晨晨[1] 余家平[1] 蒋琳[1] 徐莉莉[1] 林茂松[1] 王钰[1] 

机构地区：[1]安徽大学生命科学学院安徽省生态工程与生物技术重点实验室,合肥230039

出　　处：《植物生理学通讯》2007年第6期1101-1104,共4页Plant Physiology Communications

基　　金：安徽省科技攻关项目(03013003)

摘　　要：以大花蕙兰无菌苗假球茎为材料诱导丛生芽。适宜诱导的培养基为MS+6.4g·L^(-1)琼脂+0.5%活性炭+2.0mg·L^(-1)6- BA+0.1mg·L^(-1)NAA+30g·L^(-1)蔗糖。其芽长至2～3cm时,转入生根培养基,适宜的生根培养基为MS+6.4g·L^(-1)琼脂+ 0.2mg·L^(-1)6-BA+0.5mg·L^(-1)NAA+0.5mg·L^(-1)生根粉(ABT),生根率为100%。根长2～4cm时炼苗移栽,成活率可达95%。The fascicular buds were induced from the pseudocorm of in vitro seedlings lump of Cymbidium hybridium. The results showed that, MS＋6.4 g·L^-1 agar＋0.5% activated carbon＋2.0 mg·L^-1 6-BA＋0.1 mg·L^-1 NAA＋ 30 g·L^-1 sugar is the best medium. The buds were transferred into the rooting medium when it reached 2- 3 cm. The best rooting medium for the radication is MS＋6.4 g·L^-1 agar＋0.2 mg·L^-16-BA＋0.5 mg·L^-1NAA＋0.5 mg·L^-1 ABT. The rate of rooting could be 100%. The seedlings were planted into soil when their roots reached 2-4 cm. The survival rate amounted to 95%.

关 键 词：大花蕙兰 组织培养 丛生芽 生根培养 

分 类 号：S682.31[农业科学—观赏园艺]