地黄对“阴虚热盛”家兔血清药效指纹图谱的影响  

Experimental Research about the Effect of Rehmannia glutinosa on the Drug Effect Fingerprints of the Rabbit's Blood Serum of Yin Deficiency and Heat Flourishing Syndrome

作　　者：冯志毅[1] 崔瑛[2] 丁岗[3] 张冰[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]河南中医学院药学院,河南郑州450008 [3]江苏中康药物科技有限公司,江苏南京211100

出　　处：《中药材》2007年第12期1544-1548,共5页Journal of Chinese Medicinal Materials

基　　金：河南省自然科学基金项目(编号:011102160)

摘　　要：目的:探索地黄对阴虚热盛模型家兔血清药效指纹图谱的影响。方法:采用速尿和大肠杆菌内毒素复制家兔阴虚热盛证动物模型,通过HPLC梯度洗脱对造模、给药前后家兔血清药效指纹图谱进行研究。结果:地黄主要是影响动物自身机体代谢物的代谢水平,发挥滋阴清热作用。结论:获得的地黄"养阴清热"HPLC血清指纹图谱将有助于阐明生地黄养阴清热的药理活性及作用机理。Objective： To study about the effect of Rehmannia glutinosa Libosch on the drug effect fingerprints of the rabbit＇ s blood serum of yin deficiency and heat flourishing syndrome. Methods： The infusion of furosemide and escherichia coli endotoxin were used to replicate rabbit＇ s yin deficiency and heat flourishing syndrome animal model; high performance liquid chromatography gradient elution was adopped to study the change of the rabbit serum drug effect fingerprint in copying between before and after model-making and administration. Results ： Rehmannia glutinosa Libosch mainly affected metabolism level of the inner substance with the function of nourishing yin clearing heat. Conclusion： The serum HPLC fingerprint established is capable to reflect the function of nourishing yin clearing heat of Rehmannia glutinosa Libosch ; the further study on its drug effect fingerprint will help to clarify its activity and metabolism of action.

关 键 词：地黄 阴虚热盛 药效指纹图谱 HPLC 

分 类 号：R285.5[医药卫生—中药学]