红芪总多糖对小鼠S_(180)瘤的抑制作用及其机制研究  

Study on Depressant effects of THPS on S_(180) Tumor-bearing-mice and Its Mechanisms

作　　者：雷丰丰[1] 赵健雄[1] 王学习[1] 姚宝泰[1] 丁侃[2] 

机构地区：[1]兰州大学中西医结合研究所 [2]中国科学院上海药物研究所,上海201203

出　　处：《中药材》2007年第12期1548-1551,共4页Journal of Chinese Medicinal Materials

基　　金：甘肃省科技攻关项目(2GS064-A43-020-32);兰州大学医学科研基金(LZUYX200601)

摘　　要：目的:研究红芪总多糖(THPS)对小鼠S180瘤的抑制作用及其作用机制。方法:水提醇沉法从红芪根中提取THPS并测定其分子量、纯度和糖、糖醛酸含量及单糖组成。昆明小鼠90只随机分为9组,第1组作为正常组,另8组接种S180瘤后重新分别给予低、中、高不同剂量的THPS及THPS合环磷酰胺(CY)治疗,14 d后处死小鼠。测定每组小鼠的瘤重和抑瘤率,ELISA法测定每组小鼠外周血白介素2(IL-2)和肿瘤坏死因子α(TNF-α),免疫组化法测定肿瘤核转录因子κB(NF-κB)。结果:THPS中剂量对小鼠S180瘤有明显抑制作用,且与CY合用后有协同作用,并明显增高小鼠IL-2、TNF-α、NF-κB的含量。结论:中剂量的THPS具有抑制S180瘤作用,其机制通过增高IL-2、TNF-α和NF-κB发挥作用。Objective： to study depressant effect of total hedysarum polybotys saccharids （THPS） on S180 tumor-bearing-mice and its mechanisms. Mαhods： THPS was extracted from Radix Xedysari with water and precipitated with αhanol, dαermining its molecular weight, purity, saccharide and aldonic acid content. 90 Kunming mice were divided into 9 groups randomly. One group was the normal group, the others were divided into 8 groups randomly after inculating S180 tumors and were treated with THPS and THPS combination cyclophosphamide （CY） in low, moderate and high dose, to put them to death after 14 days. To dαermine every tumor weight, the rate of depressant tumor, the contents of IL-2 and TNF-α with ELISA, and NF-κB with immunochemistry. Results： The moderate dose THPS conspicuously possessed a depressant effect on S180 tumor and joint action with combination CY and increased the contents of IL- 2, TNF-α and NF-κB of mice. Conclusion： THPS of moderate dose possesses a depressant effect on S180 tumor through increasing the contents of IL-2, TNF-α and NF-κB of mice.

关 键 词：红芪总多糖 S180瘤 白介素2 肿瘤坏死因子Α 核转录因子ΚB 

分 类 号：R285.5[医药卫生—中药学]