低剂量混合稀土常乐对大鼠血清胰岛素及肝细胞糖原的影响  

Effect of Low Dosage Changle on Serum Insulin and Hepatocyte Glycogen in Rats

作　　者：黄可欣[1] 马洪喜[2] 石博[1] 李树蕾[3] 崔丽[1] 

机构地区：[1]吉林大学基础医学院形态学中心,吉林长春130021 [2]吉林大学第一临床医学院病理科,吉林长春130021 [3]吉林大学基础医学院组织胚胎学教研室,吉林长春130021

出　　处：《环境与健康杂志》2007年第6期403-405,共3页Journal of Environment and Health

基　　金：国家自然科学基金重大课题资助项目[29890280-3(2)]

摘　　要：目的研究长期灌胃低剂量混合稀土常乐对大鼠血清胰岛素、血糖及肝细胞糖原的影响。方法将Wistar大鼠随机分为两大组,即6月组和7月组,每组又分为对照组与混合稀土常乐组。混合稀土常乐组又分为4个剂量组:0.1、0.2、2.0、10.0mg/kg,每组30只大鼠,雌雄各半。混合稀土常乐组每日以灌胃方式给予上述不同剂量的混合稀土常乐,对照组给予等量0.9/生理盐水。6月组持续灌胃6个月,7月组于灌胃6个月后,停止给予混合稀土常乐及等量生理盐水,正常饲喂1个月。利用放射免疫分析技术和显微图像分析技术检测大鼠血清中胰岛素的水平、血糖水平和肝细胞糖原的水平。结果给予混合稀土常乐6个月后,10.0mg/kg组雌性、雄性大鼠胰岛素水平下降、血糖水平升高和肝细胞糖原的灰度值升高,与对照组比较,差异有统计学意义(P<0.05)。0.1、0.2、2.0mg/kg组雄性大鼠胰岛素水平升高、血糖水平下降和肝细胞糖原的灰度值下降,与对照组比较,差异有统计学意义(P<0.05)。灌胃6个月后,停药1个月检测,雌性、雄性大鼠各组胰岛素水平和肝细胞糖原的灰度值基本恢复正常。结论低剂量混合稀土常乐长期作用可以引起大鼠血清中胰岛素水平和肝细胞糖原发生变化,这种变化可在短期内恢复正常;较低浓度的混合稀土常乐(0.1、0.2和2.0mg/kg)可促进胰岛素分泌和肝细胞糖原合成。Objective To investigate the long-term effects of low dosage Changle intake on the serum insulin and hepatocyte glycogen of rats. Methods The levels of serum insulin and hepatocyte glycogen in rat were measured by radioimmunoassay and microscopy image analysis system after 6 and 7 months of administrated with Changle（0. 1, 0.2, 2.0 and 10.0 mg/kg）. Results Taken Changle for 6 months, the serum insulin level in the 10.0 mg/kg group reduced, hepatocyte glycogen level increased in the female and the male rats. In 0.1, 0.2, and 2.0 mg/kg groups, the serum insulin level increased and the hepatocyte glycogen level decreased in the male. After 6 months of the Changle treatment, the treatment was then followed by normal food, one month later, the level of serum insulin and hepatocyte glycogen returned to the normal level. Conclusion A long-term low dosage exposure of Changle can induce the serum insulin and hepatocyte glycogen changes in rats and the serum insulin and hepatocyte glycogen levels may return to the normal levels after a short time of stopping Changle intake.

关 键 词：金属 稀土 胰岛素 肝糖原 

分 类 号：R994.6[医药卫生—毒理学]