昆明白小鼠精原干细胞的体外培养  

In Vitro Culture of Spermatogonial Stem Cells from Kunming Mice

作　　者：王庆忠[1] 刘慧莲[1] 

机构地区：[1]潍坊学院,山东潍坊261061

出　　处：《潍坊学院学报》2007年第6期56-61,76,共7页Journal of Weifang University

摘　　要：建立了一种小鼠精原干细胞(spermatogonial stem cells,SSCs)的分离、纯化和培养体系,主要包括SSCs的差异贴壁分选(differential adherence selection)、无血清培养基和MEF(mouse embryonic fibro-blast)饲养层。无血清培养基以StemPro-34 SFM为基础培养液,补充多种添加剂和GDNF(glial cell line-derived neurotrophic factor)、可溶性GFRα1(soluble GDNF family receptorα-1)和bFGF(basic fibroblastgrowth factor)等因子。昆明白小鼠(KMmice)幼鼠的生精细胞在这一培养体系中能够培养和增殖1个月。RT-PCR检测还表明培养的细胞同时表达Oct4和Sox2,说明SSCs与ESCs两者的自我复制可能享有共同的维持机制。The system of isolation and culture on spermatogonial stem cells （SSCs） was first established. It was composed of differential adherence selection of SSCs, serum - free medium and mouse embryonic fibroblast （MEF） feeder cells. Germ cells from KM pups could be cultured on MEF feeders in StemPro -34 SFM medium supplemented with glial cell line - derived neurotrophic factor （GDNF） , soluble GDNF family receptor α - 1 （ GFRα ） and basic fibroblast growth factor （bFGF） for 1 month. Expression of Oct4 and Sox2, which are crucial factors in ESC self - renewal, were detected in our cultured SSCs, suggesting that SSCs may share some common mechanisms controlling self- renewal with ESCs.

关 键 词：精原干细胞 无血清培养基 昆明白小鼠 

分 类 号：Q492[生物学—生理学]