应用氯化锂法小量提取质粒DNA  

A new method—LiCl for extracting bacterium plasmid DNA

作　　者：潘建菁[1] 张君诚[2] 卢艺萍[1] 张杭颖[2] 庄伟建[1] 

机构地区：[1]福建农林大学作物科学学院,福建福州350002 [2]三明学院,福建三明365000

出　　处：《福建农业学报》2007年第4期469-470,共2页Fujian Journal of Agricultural Sciences

基　　金：国家烟草专卖局资助项目(110200601011)

摘　　要：用改良的氯化锂方法小量提取质粒DNA。结果表明,该方法所提取的质粒DNA的质量与常规碱裂解法获得的质粒DNA基本一致,可以满足分子生物学基础实验的要求;同时该方法简化了试验步骤,质粒DNA提取时间缩短了24 min。A new method to extract small amount of plasmid DNA with LiCl was developed. The results showed that the quality of plasmid DNA extracted by using this method was basically identical to that of the conventional alkalinelysis. The method satisfied the need for basic molecular biology experiments, and the procedure was simplified shortening the extracting time by 24 min.

关 键 词：质粒DNA 氯化锂 

分 类 号：Q-33[生物学]