亚硒酸钠对HepG2.2.15细胞凋亡的影响  

Effects of Sodium SeleniteInduced the Apoptosis of HepG2.2.15 Cell Line in Vitro

作　　者：陈显兵[1] 

机构地区：[1]湖北民族学院医学院病理教研室,湖北恩施445000

出　　处：《湖北民族学院学报（医学版）》2007年第4期10-12,共3页Journal of Hubei Minzu University(Medical Edition)

基　　金：湖北省卫生厅项目(JX3B74);湖北民族学院青年资助项目

摘　　要：目的研究亚硒酸钠对体外培养的HepG2.2.15细胞株凋亡的影响。方法采用体外培养的方法,流式细胞仪分析细胞DNA水平及细胞周期,并测定细胞凋亡情况。结果2.0mmol/L亚硒酸钠作用72 h,HE切片可见核固缩、染色质边集等改变;4mmol/L亚硒酸钠作用72 h,G0/G1期和S期细胞明显减少,在细胞周期G1前期可见典型的细胞凋亡峰。结论高剂量亚硒酸钠可诱导HepG2.2.15细胞株发生凋亡。Objective To investigate the apoptosis effect of Sodium Selenite on HePG2.2.15 cell line in vitro. Methods The G2.2.15 cell line was cultured in vitro. The content of DNA, the changes of cell cycle and the apoptosis of the cells were analyzed by flow cytometry. Results When the cells were treated with Sodium Selenite at a concentration of 2.0mmol//L lasting 72h, karyopyknosis and chromatin condensation were observed under microscope; When the cells were treated with Sodium Selenite at a concentration of 4.0mmol/L lasting 72h, the cells in G0/G1 and S phase was significantly decreased, a typical apoptosis peak before G1 phase was shown. Conclusion Sodium Selenite can induce the apoptosis of HePG2.2.15 cell line.

关 键 词：亚硒酸钠 HEPG 2.2.15细胞株 凋亡 

分 类 号：R979.1[医药卫生—药品]