MIP-1α基因转染的肿瘤细胞对免疫细胞的体内外趋化作用  

THE IN VITRO AND IN VIVO CHEMOTACTIC EFFECT ON IMMUNE EFFECTOR CELLS BY MIP 1α GENE TRANSFECTED TUMOR VACCINE

作　　者：陈国友[1] 曹雪涛[1] 雷虹[1] 何龙[1] 周正芳[1] 

机构地区：[1]第二军医大学免疫学教研室

出　　处：《免疫学杂志》1997年第3期152-155,共4页Immunological Journal

基　　金：国家自然科学基金;优秀中青年人才专项基金

摘　　要：ＭＩＰ－１α是一种对多种免疫效应细胞包括Ｔ细胞、Ｂ细胞、单核细胞及中性粒细胞等具有显著趋化作用的趋化因子。通过重组腺病毒载体将ＭＩＰ－１α基因导入Ｂ１６黑色素瘤细胞后，对其体内外生物学特性进行了初步的研究。结果表明，ＭＩＰ－１α基因转染后Ｂ１６黑色素瘤细胞体外生长特性无明显变化，其培养上清中ＭＩＰ－１α的分泌水平可达３６８±２４ｎｇ／ｍｌ／１０６／２４ｈ，上清对ＮＫ细胞、ＣＤ４＋Ｔ细胞、ＣＤ８＋Ｔ细胞及巨噬细胞均具有极强的趋化作用。ＭＩＰ－１α基因转染的肿瘤细胞接种至小鼠皮下后，肿瘤生长明显减慢，肿瘤局部有大量的炎性细胞浸润；然而荷瘤小鼠的存活期未见明显增强，其ＮＫ活性及ＣＴＬ活性也未见增强，推测其原因与体内ＭＩＰ－１α所致的非特异性炎症反应过强有关。In the present study, following the B16 melanoma cells were transfected with recombinant adenovirus containing murine macropage inflammatory protein 1α(MIP 1α) gene, the biological characterise of the MIP 1α gene transfected B16 melanoma cells were investigated. The level of MIP 1α in the supernatant of gene transfected melanoma cells was 368±24ng/ml/10 6/24h. This supernatant showed strong chematactic activity for NK cells, CD4 + T cells, CD8 + T cells or the freshly isolated peritoneal macrophages. Though the in vitro growth of the genetransfected B16 melanoma cells were not altered, the in vivo growth of the subcutaneously inoculated tumor cells were significantly inhibited. The infiltration of inflammatory cells into the tumor mass formed by gene transfected B16 cells was much more obvious than that by wild type B16 cells or B16 cells transfected with the control gene. However, the survival time of the mice bearing B16 melanoma cells transfected with MIP 1α gene was not prolonged and the NK, CTL activity was not enhanced. We suggested that the in vivo phenomenon may be related to the high toxicity of the MIP 1α as a strong non specific inflammatory mediator.

关 键 词：MIP-1Α 基因转染 肿瘤细胞 免疫细胞 

分 类 号：R392.12[医药卫生—免疫学] R730.3[医药卫生—基础医学]