当归多糖与血小板源性生长因子、血小板生成素对巨核细胞造血影响的比较研究  

Comparison of the effect of Angelica polysaccharide, platelet-derived growth factor and thrombopoietin on megakaryocytopoiesis

作　　者：莫姝[1] 于洁[1] 杨默[2] 李志光[2] 李桂霞[2] 张磊[1] 

机构地区：[1]重庆医科大学附属儿童医院血液肿瘤科,400014 [2]香港中文大学威尔斯亲王医院儿科系

出　　处：《中华儿科杂志》2008年第1期45-48,共4页Chinese Journal of Pediatrics

摘　　要：目的研究当归多糖（APS）、血小板源性生长因子（PDGF）和血小板生成素（TPO）对巨核细胞株M-07e生长和凋亡的影响作用，为进一步探讨细胞因子对巨核细胞／血小板造血的作用及其机制提供基础。方法采用细胞计数和锥虫蓝拒染法进行细胞存活率检测，Annexin Ⅴ、Caspase-3和JC-1 3种流式方法检测APS、PDGF和TPO对无血清培养诱导的M-07e凋亡的影响。结果M-07e培养24、48、72h后，APS组、PDGF组和TPO组细胞增殖及细胞存活率都显著高于对照组（P〈0．05）。Annexin Ⅴ法检测72h各组死亡细胞，APS组、PDGF组和TPO组中全部死亡细胞分别占（19．41±7．59）％、（21．38±7．25）％和（18．77±8．00）％，与对照组（34．33±5．46）％比较差异有统计学意义；Caspase-3检测结果显示，APS组、PDGF组和TPO组中Caspase-3活性增高的细胞分别占（12．27±5．18）％、（12．39±6．26）％和（13．75±8．25）％，其中APS组、PDGF组与对照组（18．92±6．09）％比较，差异均有统计学意义（P〈0．05）；JC-1检测结果显示，APS组、PDGF组、TPO组和对照组中死亡细胞分别占（23．64±6．69）％、（28．00±10．05）％、（27．99±8．99）％和（39．48±11．86）％，APS组与对照组比较差异有统计学意义（P〈0．01），APS组与PDGF组、TPO组比较，差异无统计学意义。结论APS与PDGF、TPO都具有促进M-07e增殖和抵抗无血清培养诱导的M-07e细胞凋亡的作用，且APS与PDGF、TPO的作用相当。Objective To investigate the effect of Angelica polysaccharide （APS）, platelet-derived growth factor （PDGF） and thrombopoietin （TPO） on the proliferation and apoptosis of human megakaryocytic cell line M-07e. Methods Cell count and the viability testing of M-07e cells （ trypan blue exclusion assay） were performed at 24 hours, 48 hours and 72 hours after treatment with APS, PDGF or TPO. Three apoptosis related flow cytometric assays including Annexin V, Caspase-3 and JC-1 were performed to determine apoptotic rate of each group at 72 hours after the treatment. Results After the incubation, the number of M-07e cells in the APS, PDGF and TPO group increased and the viabilities of the three groups were significantly higher than the control group （P 〈0. 05）. The dead cells in the APS, PDGF and TPO group were （ 19.41 ± 7. 59） % , （21.38 ± 7.25 ） % and （ 18.77 ± 8.00） % , respectively by flow cytometry using Annexin V method, which were significantly lower compared to the control group （34. 33 ± 5.46）%. The expression of the activated caspase-3 in the group of APS, PDGF and TPO were （ 12. 27 ± 5.18） % , （ 12. 39 ± 6. 26 ） % and （ 13.75 ± 8.25 ） % , the APS and PDGF group decreased significantly compared to the control group （ 18.92±6.09）%. The ratio of total cell deaths in the APS, PDGF and TPO group were （23.64±6.69）%, （28.00 ± 10.05）% and （27.99±8.99）%, the ratio in APS group decreased significantly compared to the control group （39. 48 ± 11.86）% by JC-1 method. Differences between APS and PDGF groups and between APS and TPO groups were not statistically significant. Conclusion APS, PDGF and TPO have similar effect in stimulating proliferation and inhibiting serum-free-culture induced apoptosis of M-07e cells.

关 键 词：血小板生成 当归 多糖类 血小板源性生长因子 血小板生成素 

分 类 号：R686[医药卫生—骨科学]