PEI-氧化铁磁性纳米颗粒作为基因载体的实验观察  

Feasibility of magnetic nanoparticles modified by PEI as gene vector

作　　者：陈道桢[1] 薛文群[1] 龚健[1] 詹惠英[1] 汤月萍[1] 吴高雄[1] 王家俊[1] 

机构地区：[1]南京医科大学附属无锡妇幼保健院中心实验室,江苏无锡214002

出　　处：《第四军医大学学报》2008年第1期13-16,共4页Journal of the Fourth Military Medical University

基　　金：江苏省自然科学基金(BK2007023);南京医科大学重点项目(06NMUZ044);无锡市医院管理中心重大技术项目(ZD0604)

摘　　要：目的:评价氧化铁磁性纳米颗粒(MNPs)作为体外基因载体的可行性及外加磁场对于其转染效率的影响.方法:以FeCl3水溶液作原料,采用部分还原沉淀法制备纳米磁性颗粒,并用聚乙烯亚胺(PEI)对其进行表面修饰.利用电镜、X-射线衍射、琼脂糖凝胶电泳等手段对MNPs的形貌、粒度分布、物相组成、DNA结合能力等进行表征.应用荧光素酶报告基因系统,检测MNPs-PEI将外源基因转染至不同细胞系的转染特性以及外加磁场对基因载体效率的影响.结果:MNPs-PEI可将外源基因转染至多个细胞系并高效表达.不同细胞系的转染效率和时间各不相同.外加磁场可使转染效率提高4～5倍.结论:PEI修饰的磁性纳米颗粒是一种可用于体外转染的新型非病毒基因载体,外加磁场可提高其转染效率.AIM： To evaluate the feasibility of magnetic nanoparticles （MNPs） as gene vector and the effect of magnetic field on efficiency of transfection. METHODS： MNPs were prepared by controlling some chemical reaction parameters through partial reduction/precipitation method with ferric chloride aqueous solutions as precursor material. The surface of particles were modified by polythylenei （PEI） agents. The appearance, the size distribution, structure and pHase constitute of MNPs were characterized by transmission electron microscopy and X-ray diffraction; the potential of adsorbing DNA of MNPs was analyzed by electrophoresis; Transfection was determined by delivering reporter gene, PGL2-control encoding luciferase, to different cell lines using MNPs-PEI as vector. The effect of magnetic field on efficiency of transfection was determined using Nd-Fe-B permanent magnet. RESULTS： Foreign gene could be delivered to various cell lines by MNPs-PEI and expressed with high efficiency, but the transfection efficiency and time course varied in the different cell lines studied. Magnetic field could enhance the efficiency of transfection by 5 - 10 folds. CONCLUSION： MNPs-PEI can be used as a novel non-viral gene vector in vitro, which offers a basis for gene delivery in vivo.

关 键 词：磁性 纳米粒子 转染 磁场 

分 类 号：TN383[电子电信—物理电子学]