单克隆天然抗角蛋白抗体IgM 3B4体外抑制白念珠菌芽管形成及粘附作用  

Inhibitory effect of natural monoclonal anti-keratin antibody IgM 3B4 on germ tube formation and adhesion of Can-dida albicans in vitro

作　　者：陈新辉[1] 赵明玄[2] 安金刚[1] 李巍[1] 刘玉峰[1] 

机构地区：[1]第四军医大学西京医院全军皮肤病研究所,陕西西安710033 [2]成都军区昆明总医院核医学科,云南昆明650032

出　　处：《第四军医大学学报》2008年第1期47-50,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30330510)

摘　　要：目的:观察单克隆天然抗角蛋白抗体IgM3B4在体外抑制白念珠菌芽管形成及抑制白念珠菌与宿主细胞粘附的作用.方法:将不同浓度的单克隆天然抗角蛋白抗体IgM3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育,倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数;将不同浓度的单克隆天然抗角蛋白抗体IgM3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育,分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数.结果:与对照组比较,不同浓度的单克隆天然抗角蛋白抗体IgM3B4均能抑制白念珠菌芽管生成,并且抗体浓度越高,抑制白念珠菌芽管形成的作用越明显;同时单克隆天然抗角蛋白抗体IgM3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附,其抑制作用与单克隆天然抗角蛋白抗体IgM3B4的浓度呈正相关.结论:单克隆天然抗角蛋白抗体IgM3B4在体外能够抑制白念珠菌芽管形成,能够抑制白念珠菌与人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞的粘附,从而降低白念珠菌的侵袭力,提示天然抗体IgM在机体抗真菌感染的天然免疫中可能具有积极的作用.AIM： To investigate inhibitory action of a natural monoclonal anti-keratin antibody IgM 3B4 specifically against Candida albicans in vitro. METHODS： The different concentrations of natural monoclonal anti-keratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoelonal anti-keratin antibody IgM 3B4 to Candida albicans germ tube formation. And the different concentrations of natural monoclonal anti-keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti-keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein. RESULTS： It was found that the different concentrations of natural monoclonal anti-keratin antibody IgM 3B4 could inhibit Candida albicans germ tube formation and adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein. Its inhibitory action was positively related to the level of natural monoclonal anti-keratin antibody IgM 3B4. CONCLUSION： Natural monoclonal anti- keratin antibody IgM 3B4 can inhibit the adhesion of Candida albicans to the malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein, and decrease the invasion of Candida albicans due to its inhibitory action on the formation of Candida albicans germ tube,which indicates that natural antibody IgM plays a role in the natural immunity against yeast infection.

关 键 词：白念珠菌 单克隆抗体 免疫 天然 

分 类 号：R392.116[医药卫生—免疫学]