杀虫剂硝虫硫磷的高效液相色谱分析  

Determination of Pestiside Xiaochongthion by High Performance Liquid Chromatograph

作　　者：吴春先[1] 王广成[1] 聂果[1] 高立明[1] 陈丙坤[1] 张忠明[1] 

机构地区：[1]四川省农药检定所,成都610041

出　　处：《西南师范大学学报（自然科学版）》2008年第1期121-125,共5页Journal of Southwest China Normal University(Natural Science Edition)

摘　　要：采用正相和反相高效液相色谱法对硝虫硫磷进行定性分析,并确定用反相高效液相色谱法对硝虫硫磷进行定量分析.反相高效液相色谱条件:使用C18反相柱和二极管阵列检测器,以乙腈+水=65+35(V/V)为流动相,柱温30℃,流速:1.2 mL/min;,检测波长220 nm,外标法对硝虫硫磷的有效成分进行定量分析.结果表明硝虫硫磷的线性关系系数为0.999 6,测定30%硝虫硫磷乳油时,标准偏差为0.19,变异系数为0.62%,平均回收率为99.84%;测定硝虫硫磷原药时,标准偏差为0.08,变异系数为0.09%,平均回收率为100.16%.An HPLC method was used to qualitatively and quantitatively analyze the active ingredient of xiaochongthion including PP-HPLC method and RP-HPLC method. RP-HPLC method was used to quanlititively analyze xiaochongthion. The condition of RP-HPLC method： The mobile phase was Acetonitrile ＋ water（65 ＋ 35, by vol）. Column temperature was 30 ℃, flow rate was 1.0 mL/min, and detection wavelength was 220 nm. The results showed that the linear correlation coefficient was 0. 999 6, the standard deviation was 0. 19 ,the variation coefficient was 0.62%, the average recovery was 99.84% when 30% Xiaochongthion EC was detected; the standard deviation was 0.08, the variation coefficient was 0.09%, the average recovery was 100. 16% when Xiaochongthion TC was detected.

关 键 词：硝虫硫磷 高效液相色谱 分析 

分 类 号：Q482.3[生物学—生理学]