六味地黄汤及其拆方对正常小鼠和SAMP8胸腺淋巴细胞分化相关基因表达的影响  

Effect of Liuwei Dihuang decoction and its decomposed recipes on gene expression related to thymocytes differentiation in normal mice and SAMP8

作　　者：毕明刚[1] 周文霞[2] 齐春会[2] 张永祥[2] 

机构地区：[1]中国医学科学院药用植物研究所,北京100094 [2]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国免疫学杂志》2008年第1期24-27,共4页Chinese Journal of Immunology

基　　金：国家重点基础研究发展规划(973)项目(G1999054401)

摘　　要：目的:研究六味地黄汤及其"三补(SB)"、"三泻(SX)"拆方对正常小鼠和快速老化模型小鼠(SAMP8)胸腺淋巴细胞分化相关的Notch信号转导通路基因表达的影响。方法:以荧光实时定量PCR方法检测Notch1、PS1、PS2、HES1等基因表达的变化。结果:口服LW(5、10、20g/kg)可明显促进正常小鼠胸腺细胞PS2、HES1基因的表达;口服LW(10g/kg)及其SB、SX拆方,可不同程度地降低SAMP8的PS2、HES1基因表达水平。结论:LW可增强正常的小鼠胸腺细胞Notch信号强度;对免疫老化的SAMP8,LW能够降低胸腺细胞Notch信号强度。Objective：To research the effect of Liuwei Dihuang decoction and its decomposed recipes on gene expression related to thymocyte differentiation in normal mice and SAMP8. Methods：The expression of Notchl, Presenilin1, Presenilin2, HES1 mRNA was detected by quantitative real-time fluorescence PCR assay. Results：After oral administation of Liuwei Dihuang ctecoction （5, 10,20 g/kg）, the expressions of Presenilin2 and HES1 gene in thymocytes of normal mice were increased. After oral administation of Liuwei Dihuang decoction （ 10 g/kg） and SB, SX, the expressions of Presenilin2 and HES1 in thymocytes of normal mice were decreased. Conclusion：It is suggested that Liuwei Dihuang Decoction can increase the Notch signal intensity in the normal mice thymo- cytes and decrease that in SAMP8 ,which is immuno-senescenced mice.

关 键 词：六味地黄汤 免疫老化 快速老化模型小鼠(SAM) 胸腺淋巴细胞亚群 实时荧光定量逆转录-聚合酶链反应 Notch信号转导通路 

分 类 号：R392[医药卫生—免疫学]