不同丙泊酚载药体系中游离药物浓度与体外溶血性的关系  

Relationship between Free Propofol Concentrations of Different Delivery Systems for Injection and in vitro Hemolytic Activities

作　　者：蔡伟惠[1] 金方[1] 

机构地区：[1]上海医药工业研究院,上海200437

出　　处：《中国医药工业杂志》2008年第1期22-27,共6页Chinese Journal of Pharmaceuticals

摘　　要：考察了静脉注射用丙泊酚脂肪乳、聚氧乙烯蓖麻油胶束、微乳、混合胶束体系中游离药物浓度与体外溶血性的关联。用HPLC法测定游离丙泊酚的浓度,建立了UV法测定溶血百分率的方法。结果表明,载药脂肪乳、聚氧乙烯蓖麻油胶束、微乳、混合胶束体系透析后游离药物浓度(μg/ml)分别为19.5±0.6、33.5±2.8、107.3±3.9、151.1±13.1,溶血百分率(%)分别为4.6±0.4、20.0±0.4、55.7±4.0、89.0±0.8,而四者空白组的溶血百分率(%)则分别为1.5、6.1、0、31。在各载药体系中,由丙泊酚引起的溶血百分率在3.1%～58%范围内与游离药物浓度具有良好的线性关系(r=0.9650)。The relationship between free propofol concentrations of lipid emulsion, Cremophor EL micelles, microemulsion and mixed micelles for injection and in vitro hemolytic activities was investigated. The concentration of free propofol was determined by HPLC. Spectrophotometric method was established to determine hemolysis. The results showed that the concentrations of free propofol in water phase of above four preparations were （19.5± 0.6）, （33.5±2.8）, （107.3±3.9） and （151.1±13.1）μg/ml, respectively. The hemolysis of these four preparations with or without drug loading were （4.6±0.4） %, （20.0±0.4） %, （55.7±4.0） %, （89.0±0.8） % and 1.5 %, 6.1%, 0, 31%, respectively. There was good linear correlation between the concentrations of free propofol and in vitro hemolysis in the range of 3.1%-58% （r=0.9650）.

关 键 词：丙泊酚 游离丙泊酚 体外溶血 

分 类 号：R944.11[医药卫生—药剂学]