肺气肿胶囊中微量盐酸克仑特罗的LC-MS/MS法测定  被引量:2

Determination of Microamount Clenbuterol Hydrochloride in Feiqizhong Capsules by LC-MS/MS

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作  者:曹玲[1] 万嵘[2] 谭力[1] 王玉[1] 

机构地区:[1]江苏省药品检验所,江苏南京210008 [2]南京工业大学理学院,江苏南京210009

出  处:《中国医药工业杂志》2008年第1期36-38,共3页Chinese Journal of Pharmaceuticals

摘  要:建立了LC-MS/MS法测定肺气肿胶囊中微量盐酸克仑特罗的含量。采用Phenomenex Luna(2)C18色谱柱,柱温30℃,流动相为甲醇-0.1%甲酸溶液(40∶60),流速0.2ml/min,以盐酸班布特罗为内标,电喷雾离子化,选择性正离子方式检测,检测离子分别为m/z277.0/202.9(盐酸克仑特罗)和m/z368.5/294.0(内标)。盐酸克仑特罗在1~200ng/ml浓度范围内线性关系良好(r=0.9992),平均回收率为98.1%,RSD为1.18%,检测限为4.4pg。A LC-MS/MS method was established for determination of microamount clenbuterol hydrochloride in Feiqizhong capsules. A Phenomenex Luna (2) C18 column was used with the mobile phase of methanol-0.1% formic acid (40 : 60), at the flow rate of 0.2ml/min. Bambuterol hydrochloride was used as the internal standard. It was applied with ESI and operated in positive mode. The detected ions were m/z 277.0/202.9 (clenbuterol) and m/z 368.5/294.0 (internal standard). It was linear in the range of 1 - 200 ng/ml. The average recovery was 98.1% with RSD of 1.18 %. The limit detection was 4.4pg.

关 键 词:肺气肿胶囊 盐酸克仑特罗 液相色谱-串联质谱 测定 

分 类 号:TQ460.72[医药卫生—药物分析学] O657.63[化学工程—制药化工]

 

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