穴居狼蛛毒中磷酸单酯酶和核酸酶的研究  

STUDY ON PHOSPHOMONOESTERASE,RNase AND DNase IN SPIDER (LYCOSA SINGORIENSIS) VENOM

作　　者：张雁[1] 刘望夷[1] 吉永华[2] 徐科[2] 

机构地区：[1]中国科学院上海生物化学研究所 [2]中国科学院上海生理研究所

出　　处：《Zoological Research》1989年第2期139-142,共4页动物学研究（英文）

摘　　要：本文分别用对硝基酚磷酸酯、3＇-[32p]标记的RNA和大肠杆菌噬菌体入DNA作底物测定了新疆产穴居狼蛛毒中与核酸代谢有关的酶,发现此毒中含有磷酸单酯酶、核糖核酸酶和脱氧核糖核酸酶。Activities of phosphomonoesterase and nucleases hydrolysing RNA and DNA were presented in a solution of the crude venom from a spider (Lycosa sing-oriensis) collected in Xinjiang Region, China. The substrates for assaying these enzymes were 4-nitrophenyl phosphate disodium salt, 3' terminally 32p-labclled RNA and nonlabelled DNA respectively. After incubation of RNA and DNA with the venom solution, the products were detected by polyacryla-mide gel or agarose gel electrophoresis.

关 键 词：穴居狼蛛毒 磷酸单酯酶 核酸酶 

分 类 号：Q959.226[生物学—动物学]