体外培养肌腱细胞增殖与碱性成纤维细胞生长因子(BFGF)的关系探讨  

Effect of Basic Fibroblast Growth Factor on Proliferation and Dose-effect Relationship of Tendon Cells in Vitro

作　　者：谢宏明[1] 倪江东[1] 宋德业[1] 

机构地区：[1]中南大学湘雅二医院骨科,湖南长沙410011

出　　处：《实用预防医学》2007年第6期1720-1723,共4页Practical Preventive Medicine

摘　　要：目的探讨不同浓度的碱性成纤维细胞生长因子(BFGF)对体外培养肌腱细胞增殖和量效关系的影响,并初步估计促增长的最佳浓度。方法免疫组化细胞定性,MTT法染色,在酶联免疫检测仪上测出不同浓度BFGF组吸光度OD均值。结果与对照组OD均值相比:1.0、2.0、5.0、10、30、40、50ng/mlBFGF组有显著性差异(P<0.05);0.5、20ng/mlBFGF组无显著性差异(P>0.05)。各组OD均值随BFGF浓度的增加,先逐渐增大(0～5.0ng/ml),达到峰值后,又逐渐减少(5.0～50ng/ml)。结论1.0、2.0、5.0、10ng/mlBFGF有促进肌腱细胞增殖作用;0.5和20ng/mlBFGF对肌腱细胞增殖无明显作用;30、40、50ng/mlBFGF有抑制肌腱细胞增殖作用。5.0ng/ml是促肌腱细胞增殖最佳BFGF浓度。Objective To study the effects of different concentration basic fibroblast growth factors （BFGFs） on proliferation and dose - effect relationship of tendon cells cultured in vitro, and to estimate the optimal BFGF concentration of promoting proliferation. Methods The groups of different BFGF concentrations were stained by MTT method and their average OD values were detected by enzyme linked immunodetection meter. Results Compared with the OD mean value of the control group, the BFGF groups of 1.0, 2.0, 5.0, 10, 30, 40, and 50 ng/ml showed significant difference （P〈0.05）, while the BFGF groups of 0,5 and 2,0 ng/ml showed no significant difference （ P 〉 0,0.5）. When BFGF concentration varied between 0- 5.0 ng/ml, the OD mean value increased gradually; while BFGF concentration exceeded .5.0- 50 ng/ml, it decreased step by step, Conclusions 1.0, 2.0, 5,0, and 10 ng/ml BFGF could promote proliferation of tendon cells, 0.5 and 20 ng/ml BFGF show no significant effect on proliferation of tendon cells, and 30, 40 and 50 ng/ml BFGF could inhibit proliferation of tendon cells. The results suggest that 5.0 ng/ml is the optimal BFGF concentration of promoting proliferation.

关 键 词：BFGF 肌腱细胞 乳兔 增殖 OD值 量效关系 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]