盐酸氢氯噻嗪分子印迹聚合物的实验研究  

Experimental Investigation on the Molecularly Imprinted Polymer Receptor for Hydrochlorothiazide

作　　者：董艳 邱岳进[2] 孙贤祥[3] 

机构地区：[1]常州市华生制药有限公司质量管理部,江苏常州213022 [2]常州出入境检验检疫局 [3]江苏工业学院

出　　处：《江苏工业学院学报》2007年第4期21-24,共4页Journal of Jiangsu Polytechnic University

摘　　要：以盐酸氢氯噻嗪为模板分子,以甲基丙烯酸甲酯(MAA)为功能单体,以三-羟甲基丙烷-三-丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,并通过实验条件的优选,合成了对氢氯噻嗪具有特异作用的分子印迹聚合物。通过测定印迹聚合物的结合量,评价了其对氢氯噻嗪的亲和力。Scatchard分析表明,在印迹聚合物中存在对氢氯噻嗪形成不同亲合力的两类结合位点,这两类不同结合位点的离解常数分别为Kd1=57.0μmol/L和Kd2=3.96 mmol/L;其最大表观结合量分别为Qmax1=5.59μmol/g和Qmax2=216.4μmol/g。A synthetic polymer selective to Hydrochlorothiazide was prepared by a non-covalently imprinted technique, using Hydrochlorothiazide as a template, methyl methacrylate as a functional monomer and tri- [-hydroxymethyl] -propan-tri-acrylic acid as a cross-linker, and methanol as a porogenic solvent. After extraction of template, affinity of the polymer imprinted with Hydrochlorothiazide was examined in re-binding experiment. From the Scatchard analysis, binding site heterogeneity is obvious and two types of binding sites can be identified. The equilibrium dissociation constant Ke,, the apparent maximum amount Qmax1 and the binding constant Ka1 of the higher affinity binding sites can be calculated to be 57.0μmol/L, 5.59μmol/g, and 1.75 × 10^4 （mol/L）^-1 ; while Kd2 , Qmxa2, and Ka2 of the lower affinity binding sites were calculated to be 3.96 mmol/L, 216.4μmol/g and 2.53×10^2 （mol/L）^-1, respectively.

关 键 词：盐酸氢氯噻嗪 分子印迹聚合物 印迹条件 

分 类 号：Q632[生物学—生物物理学] R971[医药卫生—药品]