小麦耐1，2，4-三氯苯胁迫基因型的苗期筛选  

Screening of Tolerant Wheat Genotypes to 1, 2, 4-Trichlorobenzene Stress at Seedling Stage

作　　者：张国良[1,2] 陈文军[1] 戴其根[1] 孙国荣[1] 孙洁[3] 陶金飞[3] 严林锋[3] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室 [2]淮阴工学院生命科学与化学工程学院,江苏淮安223001 [3]淮阴工学院生命科学与化学工程学院

出　　处：《农业环境科学学报》2008年第1期30-34,共5页Journal of Agro-Environment Science

基　　金：国家“十一五”科技支撑计划重大项目(2006BAD02A03)

摘　　要：为了评定不同小麦基因型对1,2,4-三氯苯(TCB)的耐性强弱,确定合适的筛选指标和筛选浓度,采用发芽试验和砂培小麦幼苗的方法,以0(对照)、3和6mmol·kg-1沙3个浓度的TCB处理15个小麦基因型,以发芽势、发芽率、最长根长、根数、株高、芽鞘长、地上部干重、根干重和总干重的性状相对值(处理测定值/对照测定值×100%)作为幼苗耐性指数(tolerance index,TI)。基于耐性指数对各基因型进行聚类分析,将15个小麦基因型聚为耐性、较耐、较敏感和敏感4类。结果表明,虽然选用的两个胁迫浓度都可以用于基因型耐性鉴定,而且鉴定结果相似,但3mmol·kg-1沙更为合适。In order to evaluate the tolerance of fifteen wheat varieties to 1,2,4-trichlorobenzene （1,2,4-TCB） stress and identify proper screening indices and concentration, the effects of 1,2,4-TCB on the wheat seed germination and seedling were studied. The concentrations of 1,2,4-TCB were 0, 3 and 6 mmol. kg^-1 sand, and 1,2,4-TCB dissolved in 0.5 mL acetone were added into sands. Germination potential, germination rate, the longest root length, root number, plant height, coleoptile length, the dry weight of shoot and roots of different wheat varieties at seedling stage were measured. Character relative values were got from measured values of treated groups and control groups, and were used as tolerance indices （TI） of early seedlings. Cluster analysis of different wheat varieties based on TI was adopted. The results showed that the fifteen wheat varieties could be grouped into four types, .i.e. TCB sensitive varieties （including BL0207 and Yangmai 16）, relatively sensitive varieties（including Zhenyumai029 and Huai0208）, tolerant varleties（including Zhen9023, Huai0303, Huamail, Neixiang188-36, Wanmai19, Ningmail3 and Zoumai） and relatively tolerant varieties（including Yanfu188, Wenmai8, Huaimai18 and Huaimai19）. Though the two concentrations of 1,2,4-TCB used may be adopted to evaluate different wheat varieties and the result was similar, the concentration of 3 mmol. kg^-1 sand was better.

关 键 词：小麦 基因型 TCB胁迫 耐性评定 

分 类 号：Q945.78[生物学—植物学]